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Cyclic AMP-dependent osmoregulation of crp gene expression in Escherichia coli.
Umeå universitet, Medicinsk fakultet, Molekylärbiologi (Medicinska fakulteten). (Johansson)
Umeå universitet, Medicinsk fakultet, Molekylärbiologi (Medicinska fakulteten). (Uhlin)
2006 (Engelska)Ingår i: J Bacteriol, ISSN 0021-9193, Vol. 188, nr 16, s. 5935-44Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

We have found that the cyclic AMP (cAMP) receptor protein (CRP)-cAMP regulatory complex in Escherichia coli is subject to osmoregulation at the level of crp gene expression. This osmoregulation was lost in a cya mutant strain but could be restored by external addition of cAMP, suggesting that the intracellular level of cAMP is a key factor in the osmoregulation of CRP. The ability of the cell to maintain optimal CRP activity was essential for the growth and survival of the bacteria under low-osmolarity conditions as shown by studies with different crp mutant alleles. A suppressor mutant with a novel amino acid substitution (L124R) in CRP showed restored growth at low osmolarity. CRP(L124R) was not activated by cAMP and was shown to be dominant negative over the wild type. Our findings suggest that the fine-tuning of the CRP activity may be critical for bacterial viability and adaptability to changing osmotic conditions.

Ort, förlag, år, upplaga, sidor
2006. Vol. 188, nr 16, s. 5935-44
Nyckelord [en]
Cyclic AMP/*metabolism, Escherichia coli/*metabolism, Escherichia coli Proteins/genetics/*metabolism, Gene Expression Regulation; Bacterial, Receptors; Cell Surface/genetics/*metabolism, Transcription Factors/genetics/*metabolism, Water-Electrolyte Balance/*physiology
Identifikatorer
URN: urn:nbn:se:umu:diva-16474PubMedID: 16885462OAI: oai:DiVA.org:umu-16474DiVA, id: diva2:156147
Tillgänglig från: 2007-10-03 Skapad: 2007-10-03 Senast uppdaterad: 2018-06-09Bibliografiskt granskad

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Johansson, JörgenUhlin, Bernt Eric

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