Umeå universitets logga

umu.sePublikationer
Ändra sökning
RefereraExporteraLänk till posten
Permanent länk

Direktlänk
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annat format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annat språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf
Undersökning av SOQ1-HAD domänens funktion i qH. Investigation of SOQ1-HAD domain function in qH.
Umeå universitet, Teknisk-naturvetenskapliga fakulteten, Institutionen för fysiologisk botanik. (Alizée Malnoë)
2021 (Engelska)Självständigt arbete på avancerad nivå (masterexamen), 40 poäng / 60 hpStudentuppsats (Examensarbete)
Abstract [en]

Excess of sunlight damages the photosynthetic machinery and leads to photoinhibition, limiting the photosynthetic activity and plant growth and development. This excess of light energy is used or dis-sipated through photoprotective mechanisms such as emission of fluorescence or non-photochemical quenching (NPQ) of chlorophyll fluorescence. Suppressor Of Quenching 1 (SOQ1) prevents energy dis-sipation during non-stress condition but its mechanism of action is yet to be determined. To better understand SOQ1 activity, the function of its haloacid dehalogenase-like hydrolase (HAD) domain lo-cated on the stromal side, is investigated during this project. The stromal domain is not required for SOQϭ͛s fuŶĐtioŶ but a possible positive regulation of the SOQ1-HAD domain on qH has not been stud-ied yet and leaves open the question of the role of the HAD domain. Because of SOQ1 domains loca-tion, at both sides of the thylakoid membrane and its putative sugar phosphatase function, SOQ1-HAD domain could act as a metabolic sensor on the stromal side, inducing a regulation on the lumenal side. This regulation could adjust light harvesting according to the cell metabolic needs. To further investi-gate these hypotheses, several aspects around SOQ1-HAD domain have been studied during this pro-ject. First, the role of the HAD domain in SOQ1 function has been studied by fluorescence measure-ment of different mutant and overexpressor lines. The results obtained showed that SOQ1-HAD do-main may not be required for qH inhibition but could be important in SOQ1 regulation. Then, SOQ1 RNA processing has been investigated and the accumulation of SOQ1 transcript isoforms has been quantified under non-stress and stress conditions. An accumulation of SOQ1 variant containing only the HAD domain was observed and especially under stress condition. Finally, to explore the hypothesis of the metabolic sensor, phosphate-sugars have been extracted and analysed by mass spectrometry, but SOQ1-HAD domain in vivo substrate remains unknown.

Ort, förlag, år, upplaga, sidor
2021. , s. 36
Nationell ämneskategori
Bioteknologi med applikationer på växter och djur
Identifikatorer
URN: urn:nbn:se:umu:diva-185550OAI: oai:DiVA.org:umu-185550DiVA, id: diva2:1575673
Utbildningsprogram
Masterprogrammet i växt- och skogsbioteknik
Handledare
Tillgänglig från: 2021-07-01 Skapad: 2021-06-30 Senast uppdaterad: 2021-07-01Bibliografiskt granskad

Open Access i DiVA

Fulltext saknas i DiVA

Av organisationen
Institutionen för fysiologisk botanik
Bioteknologi med applikationer på växter och djur

Sök vidare utanför DiVA

GoogleGoogle Scholar

urn-nbn

Altmetricpoäng

urn-nbn
Totalt: 78 träffar
RefereraExporteraLänk till posten
Permanent länk

Direktlänk
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annat format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annat språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf