Umeå University's logo

umu.sePublications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
qRT-PCR analysis of CEACAM5, KLK6, SLC35D3, MUC2 and POSTN in colon cancer lymph nodes: An improved method for assessment of tumor stage and prognosis
Department of Clinical Sciences, Lund University, Helsingborg, Sweden; Specialistläkarna, Malmö, Sweden.
HiloProbe AB, Umeå, Sweden.
Umeå University, Faculty of Medicine, Department of Clinical Microbiology. Umeå University, Faculty of Medicine, Department of Radiation Sciences, Oncology.ORCID iD: 0000-0001-8803-4798
Umeå University, Faculty of Medicine, Department of Clinical Microbiology.
Show others and affiliations
2024 (English)In: International Journal of Cancer, ISSN 0020-7136, E-ISSN 1097-0215, Vol. 154, no 3, p. 573-584Article in journal (Refereed) Published
Abstract [en]

One fourth of colorectal cancer patients having curative surgery will relapse of which the majority will die. Lymph node (LN) metastasis is the single most important prognostic factor and a key factor when deciding on postoperative treatment. Presently, LN metastases are identified by histopathological examination, a subjective method analyzing only a small LN volume and giving no information on tumor aggressiveness. To better identify patients at risk of relapse we constructed a qRT-PCR test, ColoNode, that determines levels of CEACAM5, KLK6, SLC35D3, MUC2 and POSTN mRNAs. Combined these biomarkers estimate the tumor cell load and aggressiveness allocating patients to risk categories with low (0, −1), medium (1), high (2) and very high (3) risk of recurrence. Here we present result of a prospective, national multicenter study including 196 colon cancer patients from 8 hospitals. On average, 21 LNs/patient, totally 4698 LNs, were examined by both histopathology and ColoNode. At 3-year follow-up, 36 patients had died from colon cancer or lived with recurrence. ColoNode identified all patients that were identified by histopathology and in addition 9 patients who were undetected by histopathology. Thus, 25% of the patients who recurred were identified by ColoNode only. Multivariate Cox regression analysis proved ColoNode (1, 2, 3 vs 0, −1) as a highly significant risk factor with HR 4.24 [95% confidence interval, 1.42-12.69, P =.01], while pTN-stage (III vs I/II) lost its univariate significance. In conclusion, ColoNode surpassed histopathology by identifying a significantly larger number of patients with future relapse and will be a valuable tool for decisions on postoperative treatment.

Place, publisher, year, edition, pages
John Wiley & Sons, 2024. Vol. 154, no 3, p. 573-584
Keywords [en]
colon cancer, ColoNode, lymph nodes, prognosis, tumor markers
National Category
Cancer and Oncology
Identifiers
URN: urn:nbn:se:umu:diva-214620DOI: 10.1002/ijc.34718ISI: 001067494700001PubMedID: 37700602Scopus ID: 2-s2.0-85170666995OAI: oai:DiVA.org:umu-214620DiVA, id: diva2:1800523
Funder
Region VästerbottenSwedish Cancer SocietySwedish Research Council, 2017-00675The Kempe FoundationsUmeå UniversityVinnovaAvailable from: 2023-09-27 Created: 2023-09-27 Last updated: 2024-01-12Bibliographically approved

Open Access in DiVA

fulltext(4545 kB)63 downloads
File information
File name FULLTEXT02.pdfFile size 4545 kBChecksum SHA-512
e20335c040d160dd492637c99a048ddd9a551081cbab8912c91c4b43f0e0b7b0281ca7f68eaa991d456259b91186e0444f132b548b1b18b31db50146ed9c0888
Type fulltextMimetype application/pdf

Other links

Publisher's full textPubMedScopus

Authority records

Sitohy, BaselIsraelsson, AnneRoshdy, TamerKung, Chih-HanRutegård, MartinHammarström, StenHammarström, Marie-Louise

Search in DiVA

By author/editor
Sitohy, BaselIsraelsson, AnneRoshdy, TamerKung, Chih-HanRutegård, MartinHammarström, StenHammarström, Marie-Louise
By organisation
Department of Clinical MicrobiologyOncologySurgeryWallenberg Centre for Molecular Medicine at Umeå University (WCMM)
In the same journal
International Journal of Cancer
Cancer and Oncology

Search outside of DiVA

GoogleGoogle Scholar
Total: 91 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

doi
pubmed
urn-nbn

Altmetric score

doi
pubmed
urn-nbn
Total: 322 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf