Umeå University's logo

umu.sePublications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Pseudomonas syringae infectivity correlates to altered transcript and metabolite levels of Arabidopsis mediator mutants
Umeå University, Faculty of Medicine, Department of Medical Biochemistry and Biophysics.
Umeå University, Faculty of Science and Technology, Department of Physics. Biostatistics, School of Public Health and Community Medicine, Gothenburg University, Gothenburg, Sweden.
Umeå University, Faculty of Science and Technology, Department of Physics.
Umeå University, Faculty of Medicine, Department of Medical Biochemistry and Biophysics.
Show others and affiliations
2024 (English)In: Scientific Reports, E-ISSN 2045-2322, Vol. 14, no 1, article id 6771Article in journal (Refereed) Published
Abstract [en]

Rapid metabolic responses to pathogens are essential for plant survival and depend on numerous transcription factors. Mediator is the major transcriptional co-regulator for integration and transmission of signals from transcriptional regulators to RNA polymerase II. Using four Arabidopsis Mediator mutants, med16, med18, med25 and cdk8, we studied how differences in regulation of their transcript and metabolite levels correlate to their responses to Pseudomonas syringae infection. We found that med16 and cdk8 were susceptible, while med25 showed increased resistance. Glucosinolate, phytoalexin and carbohydrate levels were reduced already before infection in med16 and cdk8, but increased in med25, which also displayed increased benzenoids levels. Early after infection, wild type plants showed reduced glucosinolate and nucleoside levels, but increases in amino acids, benzenoids, oxylipins and the phytoalexin camalexin. The Mediator mutants showed altered levels of these metabolites and in regulation of genes encoding key enzymes for their metabolism. At later stage, mutants displayed defective levels of specific amino acids, carbohydrates, lipids and jasmonates which correlated to their infection response phenotypes. Our results reveal that MED16, MED25 and CDK8 are required for a proper, coordinated transcriptional response of genes which encode enzymes involved in important metabolic pathways for Arabidopsis responses to Pseudomonas syringae infections.

Place, publisher, year, edition, pages
Springer Nature, 2024. Vol. 14, no 1, article id 6771
National Category
Biochemistry and Molecular Biology
Identifiers
URN: urn:nbn:se:umu:diva-222861DOI: 10.1038/s41598-024-57192-xPubMedID: 38514763Scopus ID: 2-s2.0-85188349282OAI: oai:DiVA.org:umu-222861DiVA, id: diva2:1851679
Funder
Knut and Alice Wallenberg Foundation, 2015-0056Swedish Foundation for Strategic Research, SB16-0089Swedish Research Council, 2016-03943Swedish Research Council, 2016-00796Available from: 2024-04-15 Created: 2024-04-15 Last updated: 2024-04-15Bibliographically approved

Open Access in DiVA

fulltext(4793 kB)37 downloads
File information
File name FULLTEXT01.pdfFile size 4793 kBChecksum SHA-512
70100f48278df8fbc2a48f9acc26aa5cdda68f0883676b579cc90bc3fb74ffe3a34369d20a8e65bec251622cf570b74ac6674d314e013876006100f8c979c74d
Type fulltextMimetype application/pdf

Other links

Publisher's full textPubMedScopus

Authority records

Blomberg, JeanetteVergara, AlexanderKaramat, FazeelatImran, Qari MuhammadStrand, ÅsaRosvall, MartinBjörklund, Stefan

Search in DiVA

By author/editor
Blomberg, JeanetteVergara, AlexanderKaramat, FazeelatImran, Qari MuhammadStrand, ÅsaRosvall, MartinBjörklund, Stefan
By organisation
Department of Medical Biochemistry and BiophysicsDepartment of PhysicsDepartment of Plant PhysiologyUmeå Plant Science Centre (UPSC)
In the same journal
Scientific Reports
Biochemistry and Molecular Biology

Search outside of DiVA

GoogleGoogle Scholar
Total: 37 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

doi
pubmed
urn-nbn

Altmetric score

doi
pubmed
urn-nbn
Total: 257 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf