umu.sePublikasjoner
Endre søk
RefereraExporteraLink to record
Permanent link

Direct link
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annet format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annet språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf
Cytolethal distending toxin upregulates RANKL expression in Jurkat T-cells: Cdt upregulates RANKL
Umeå universitet, Medicinsk fakultet, Odontologi, Parodontologi. Umeå universitet, Medicinsk fakultet, Medicinsk biovetenskap, Patologi. (Aa-gruppen)
Umeå universitet, Medicinsk fakultet, Odontologi.
2008 (engelsk)Inngår i: Acta Pathologica, Microbiologica et Immunologica Scandinavica (APMIS), ISSN 0903-4641, E-ISSN 1600-0463, Vol. 116, nr 6, s. 499-506Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

Cytolethal distending toxin, a bacterial exotoxin produced by a number of Gram-negative species, causes growth arrest and morphological alterations in host cells. Among these species are Haemophilus ducreyi, the etiological agent of chancroid, and the periodontal pathogen Aggregatibacter actinomycetemcomitans, highly implicated in localized aggressive periodontitis. CDT induces receptor activator of NF-kappaB ligand (RANKL) expression in periodontal fibroblasts, the key bone-resorbing cytokine. T-cells are actively involved in localized inflammation-induced bone destruction, including periodontitis. The aim of this study was to investigate the effects of purified CDT on the expression of RANKL and its decoy receptor osteoprotegerin (OPG), in the Jurkat T-cell line. Quantitative real-time PCR indicated that 100 pg/ml of purified H. ducreyi CDT upregulated RANKL mRNA expression by 2.2-fold, after 24 h of exposure. This increase was corroborated by a 2.0-fold increase in RANKL protein release, as determined by ELISA. OPG was not detected in this experimental system. In conclusion, CDT enhances RANKL expression in T-cells, denoting that these cells are a potential target for the toxin and strengthening the potential link between this virulence factor and mechanisms associated with localized bone resorption.

sted, utgiver, år, opplag, sider
Copenhagen: WILEY Inter Science , 2008. Vol. 116, nr 6, s. 499-506
Emneord [en]
Cdt, RANKL
HSV kategori
Forskningsprogram
mikrobiologi
Identifikatorer
URN: urn:nbn:se:umu:diva-20551DOI: 10.1111/j.1600-0463.2008.01017.xOAI: oai:DiVA.org:umu-20551DiVA, id: diva2:208903
Prosjekter
Virulence mechanisms of Aggregatibacter actinomycetemcomitansTilgjengelig fra: 2009-03-21 Laget: 2009-03-21 Sist oppdatert: 2018-06-09

Open Access i DiVA

Fulltekst mangler i DiVA

Andre lenker

Forlagets fulltekst

Personposter BETA

Brage, MonicaJohansson, Anders

Søk i DiVA

Av forfatter/redaktør
Brage, MonicaJohansson, Anders
Av organisasjonen
I samme tidsskrift
Acta Pathologica, Microbiologica et Immunologica Scandinavica (APMIS)

Søk utenfor DiVA

GoogleGoogle Scholar

doi
urn-nbn

Altmetric

doi
urn-nbn
Totalt: 636 treff
RefereraExporteraLink to record
Permanent link

Direct link
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annet format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annet språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf