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Functionalization of epoxy-based monoliths for ion exchange chromatography of proteins
Umeå universitet, Teknisk-naturvetenskapliga fakulteten, Kemiska institutionen.
Umeå universitet, Teknisk-naturvetenskapliga fakulteten, Kemiska institutionen.
Umeå universitet, Teknisk-naturvetenskapliga fakulteten, Kemiska institutionen.
Umeå universitet, Teknisk-naturvetenskapliga fakulteten, Kemiska institutionen.
Vise andre og tillknytning
2009 (engelsk)Inngår i: Journal of Separation Science, Vol. 32, nr 15-16, s. 2556-2564Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

Macroporous epoxy-based monoliths prepared by emulsion polymerization have been modified for use in ion exchange chromatography (IEC) of proteins. Strong anion exchange functionality was established by iodomethane quaternization of tertiary amine present on the monolith surface as a part of the polymer backbone. The modification pathway to cation exchange materials was via incorporation of glycidyl methacrylate (GMA) brushes which were coated using atom transfer radical polymerization (ATRP). Strong (SO3-) and weak (COO-) cation exchange groups were thereafter introduced onto the GMA-grafted monoliths by reactions with sodium hydrogen sulfite and iminodiacetic acid, respectively. Grafting was confirmed by XPS, gravimetric measurement, and chromatographic behavior of the modified materials toward model proteins. In incubation experiments the proteins were recovered quantitatively with no obvious signs of unfolding after contact with the stationary phase for >2 h. Chromatographic assessments on the functionalized columns as well as problems associated with flow-through modification by ATRP are discussed.

sted, utgiver, år, opplag, sider
Wiley InterScience , 2009. Vol. 32, nr 15-16, s. 2556-2564
Emneord [en]
ATRP, Epoxy-amine, Ion exchange chromatography, Monolithic sorbents, Protein separation
HSV kategori
Forskningsprogram
analytisk kemi
Identifikatorer
URN: urn:nbn:se:umu:diva-20889DOI: 10.1002/jssc.200900243OAI: oai:DiVA.org:umu-20889DiVA, id: diva2:209818
Tilgjengelig fra: 2009-03-27 Laget: 2009-03-27 Sist oppdatert: 2018-06-09bibliografisk kontrollert
Inngår i avhandling
1. New approaches to preparation of macroporous monoliths for use in liquid chromatography
Åpne denne publikasjonen i ny fane eller vindu >>New approaches to preparation of macroporous monoliths for use in liquid chromatography
2009 (engelsk)Doktoravhandling, med artikler (Annet vitenskapelig)
Abstract [en]

High performance liquid chromatography (HPLC) is one of the major techniques in separat-ion sciences. Faster separation and higher efficiency are required to meet ever-growing demands. Despite numerous studies and achievements on improving mass transfer in particulate packings discontinuity seems to be the cornerstone drawback in their development. Macroporous continuous beds or monoliths are therefore a promising alternative to the particle medium. This thesis deals with preparation of new monoliths used as carrier for HPLC. Two different approaches were developed for two polymer systems. One was based on polycondensation of epoxy resins and polyamines which were components of an oil-in-water emulsion. An epoxy resin mixture was dispersed in aqueous polyamine phase with the aid of a surfactant. The other involved a traverse of a ready-made polymer solution around its upper critical solution temperature (UCST). In other words, linear polyamides, non-covalently crosslinked polymers, dissolved in a solvent at temperature higher than their UCST followed by slow cooling to below the critical temperature to precipitate the polymers. Partly re-established hydrogen bonds resulted in the formation of crystallites that interconnected into a network structure. Factors controlling morphology and porosity of final products were investigated. The study also deals with surface modifying for chromatographic applications. Functionalization pathways attempted in the thesis were quaterization of inherent amine of the epoxy-based monoliths and grafting tentacle ion groups via glycidyl methacrylate by atom transfer radical polymerization (ATRP) for ion exchange chromatography (IEC).

sted, utgiver, år, opplag, sider
Umeå: Umeå University, Department of Chemistry, 2009. s. 53
Emneord
monolith, polycondensation, dissolution-precipitation, epoxy-amine, poly-amide, nylon, emulsion polymerization, characterization, protein separation, liquid chromatography
HSV kategori
Forskningsprogram
analytisk kemi
Identifikatorer
urn:nbn:se:umu:diva-20890 (URN)978-91-7264-772-5 (ISBN)
Utgiver:
Kemiska institutionen, 90187, Umeå
Disputas
2009-04-17, KB3A9, Umeå University, Chemistry, Umeå, 13:00 (engelsk)
Opponent
Veileder
Tilgjengelig fra: 2009-03-30 Laget: 2009-03-27 Sist oppdatert: 2018-06-09bibliografisk kontrollert

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