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N-acetylcysteine alters apoptotic gene expression in axotomised primary sensory afferent subpopulations.
Umeå universitet, Medicinska fakulteten, Institutionen för integrativ medicinsk biologi (IMB), Anatomi. Umeå universitet, Medicinska fakulteten, Institutionen för kirurgisk och perioperativ vetenskap, Handkirurgi.
Umeå universitet, Medicinska fakulteten, Institutionen för integrativ medicinsk biologi (IMB), Anatomi. Umeå universitet, Medicinska fakulteten, Institutionen för kirurgisk och perioperativ vetenskap, Handkirurgi.
Umeå universitet, Medicinska fakulteten, Institutionen för integrativ medicinsk biologi (IMB), Anatomi. Umeå universitet, Medicinska fakulteten, Institutionen för kirurgisk och perioperativ vetenskap, Handkirurgi.
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2009 (engelsk)Inngår i: Neuroscience research, ISSN 0168-0102, E-ISSN 1872-8111, Vol. 65, nr 2, s. 148-155Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

Novel approaches are required in peripheral nerve injury management because current surgical techniques, which do not address axotomy-induced neuronal death, lead to deficient sensory recovery. Sensory neuronal death has functional preference with cutaneous neurons dying in great numbers whilst muscle afferents survive axotomy. This offers the potential of comparing similar cell types that suffer distinct fates upon nerve injury. Here, a novel approach, combining in vivo rat nerve injury model with laser microdissection and quantitative real-time polymerase chain reaction, identifies crucial disparities in apoptotic gene expression attributable to subpopulations of differing sensory modalities and examines the response to N-acetylcysteine (NAC) therapy. We show that axotomised muscle afferent neurons survive injury due to a neuroprotective response which markedly downregulates Bax and caspase-3 mRNA. In contrast, axotomised cutaneous sensory neurons significantly upregulate caspase-3 and alter both Bcl-2 and Bax expression such that pro-apoptotic Bax predominates. N-Acetylcysteine (NAC) intervention promotes neuroprotection of cutaneous sensory neurons through considerable upregulation of Bcl-2 and downregulation of both Bax and caspase-3 mRNA. The data presented identifies differential activation of apoptotic genes in axotomised neuronal subpopulations. Furthermore, NAC therapy instigates apoptotic gene expression changes in axotomised neurons, thereby offering pharmacotherapeutic potential in the clinical treatment of nerve injury.

sted, utgiver, år, opplag, sider
2009. Vol. 65, nr 2, s. 148-155
Emneord [en]
Peripheral nerve injury, Dorsal root ganglion, Neuronal apoptosis, Neuroprotection, Rat
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Identifikatorer
URN: urn:nbn:se:umu:diva-32017DOI: 10.1016/j.neures.2009.06.008PubMedID: 19559059OAI: oai:DiVA.org:umu-32017DiVA, id: diva2:301283
Tilgjengelig fra: 2010-03-03 Laget: 2010-02-26 Sist oppdatert: 2018-06-08bibliografisk kontrollert

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