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Initiator methionine tRNA is essential for Ty1 transposition in yeast
Umeå universitet, Medicinska fakulteten, Mikrobiologi. Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, 725 North Wolfe Street, Baltimore, MD.
Umeå universitet, Teknisk-naturvetenskapliga fakulteten, Institutionen för molekylärbiologi (Teknisk-naturvetenskaplig fakultet).
1992 (engelsk)Inngår i: Proceedings of the National Academy of Sciences of the United States of America, ISSN 0027-8424, E-ISSN 1091-6490, Vol. 89, nr 8, s. 3236-3240Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

The yeast retrotransposon Ty1 transposes through an RNA intermediate by a mechanism similar to that of retroviral reverse transcription and integration. Ty1 RNA contains a putative minus strand primer binding site (-PBS) that is complementary to the 3' acceptor stem of the initiator methionine tRNA (tRNA(iMet)). Here we demonstrate that the tRNA(iMet) is used as a primer for Ty1 reverse transcription. Mutations in the Ty1 element that alter 5 of 10 nucleotides that are complementary to the tRNA(iMet) abolish Ty1 transposition, even though they are silent with regard to Ty1 protein coding. We have constructed a yeast strain lacking wild-type tRNA(iMet) that is dependent on a mutant derivative of tRNA(iMet) that has an altered acceptor stem sequence, engineered to restore homology with the Ty1 -PBS mutant. The compensatory mutations made in the tRNA(iMet) alleviate the transposition defect of the Ty1 -PBS mutant. The mutant and wild-type tRNA(iMet) are enriched within Ty1 virus-like particles irrespective of complementarity to the Ty1 -PBS. Thus, complementarity between the Ty1 -PBS and tRNA(iMet) is essential for transposition but is not necessary for packaging of the tRNA inside virus-like particles.

sted, utgiver, år, opplag, sider
National Academies Press, 1992. Vol. 89, nr 8, s. 3236-3240
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URN: urn:nbn:se:umu:diva-32177DOI: 10.1073/pnas.89.8.3236PubMedID: 1314382OAI: oai:DiVA.org:umu-32177DiVA, id: diva2:301473
Tilgjengelig fra: 2010-03-03 Laget: 2010-03-03 Sist oppdatert: 2019-01-23bibliografisk kontrollert

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