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Neurotransmitter synthesis in poststroke cortical neurogenesis in adult rats.
Umeå universitet, Medicinska fakulteten, Institutionen för folkhälsa och klinisk medicin, Medicin. Umeå universitet, Medicinska fakulteten, Institutionen för farmakologi och klinisk neurovetenskap, Neurologi.
Umeå universitet, Medicinska fakulteten, Institutionen för folkhälsa och klinisk medicin, Medicin.
Umeå universitet, Medicinska fakulteten, Institutionen för folkhälsa och klinisk medicin, Medicin.
Umeå universitet, Medicinska fakulteten, Institutionen för folkhälsa och klinisk medicin, Medicin.ORCID-id: 0000-0003-0394-5096
2010 (Engelska)Ingår i: Stem cell research, ISSN 1876-7753, Vol. 4, nr 2, s. 148-154Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

Neurogenesis occurs in the cerebral cortex of adult rats after focal cerebral ischemia. Whether or not the newborn neurons could synthesize neurotransmitters is unknown. To elucidate such a possibility, a photothrombotic ring stroke model with spontaneous reperfusion was induced in adult male Wistar rats. The DNA duplication marker BrdU was repeatedly injected, and the rats were sacrificed at various times after stroke. To detect BrdU nuclear incorporation and various neurotransmitters, brain sections were processed for single/double immunocytochemistry and single/double/triple immunofluorescence. Stereological cell counting was performed to assess the final cell populations. At 48 h, 5 days, 7 days, 30 days, 60 days and 90 days after stroke, numerous cells were BrdU-immunolabeled in the penumbral cortex. Some of these were doubly immunopositive to the cholinergic neuron-specific marker ChAT or GABAergic neuron-specific marker GAD. As analyzed by 3-D confocal microscopy, the neurotransmitters acetylcholine and GABA were colocalized with BrdU in the same cortical cells. In addition, GABA was colocalized with the neuron-specific marker Neu N in the BrdU triple-immunolabeled cortical cells. This study suggests that the newborn neurons are capable of synthesizing the neurotransmitters acetylcholine and GABA in the penumbral cortex, which is one of the fundamental requisites for these neurons to function in the poststroke recovery.

Ort, förlag, år, upplaga, sidor
Elsevier , 2010. Vol. 4, nr 2, s. 148-154
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Medicin och hälsovetenskap
Identifikatorer
URN: urn:nbn:se:umu:diva-35682DOI: 10.1016/j.scr.2009.12.001ISI: 000276297600007PubMedID: 20089468OAI: oai:DiVA.org:umu-35682DiVA, id: diva2:346190
Tillgänglig från: 2010-08-31 Skapad: 2010-08-31 Senast uppdaterad: 2018-06-08Bibliografiskt granskad

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