umu.sePublikasjoner
Endre søk
RefereraExporteraLink to record
Permanent link

Direct link
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annet format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annet språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf
Diclofenac in fish: blood plasma levels similar to human therapeutic levels affect global hepatic gene expression
Sahlgrenska Academy at the University of Gothenburg, Göteborg, Sweden.
Sahlgrenska Academy at the University of Gothenburg, Göteborg, Sweden och Chalmers University of Technology and University of Gothenburg, Göteborg, Sweden.
Umeå universitet, Teknisk-naturvetenskapliga fakulteten, Kemiska institutionen.
Sahlgrenska Academy at the University of Gothenburg, Göteborg, Sweden och Faculty of Science, University of Gothenburg, Göteborg, Sweden.
Vise andre og tillknytning
2011 (engelsk)Inngår i: Environmental Toxicology and Chemistry, ISSN 0730-7268, E-ISSN 1552-8618, Vol. 30, nr 9, s. 2126-2134Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

Diclofenac is a non-steroidal anti-inflammatory drug frequently found in the aquatic environment. Previous studies have reported histological changes in the liver, kidney and gills of fish at concentrations similar to those measured in treated sewage effluents (approximately 1 µg/L). Analyses or predictions of blood plasma levels in fish allow a direct comparison with human therapeutic plasma levels, and may therefore be used to indicate a risk for pharmacological effects in fish. To relate internal exposure to a pharmacological interaction we investigated global hepatic gene expression together with bioconcentration in blood plasma and liver of rainbow trout (Oncorhynchus mykiss) exposed to waterborne diclofenac. At the highest exposure concentration (81.5 µg/L) the fish plasma concentration reached approximately 88% of the human therapeutic levels (C(max) ) after two weeks. Using an oligonucleotide microarray followed by quantitative PCR we found extensive effects on hepatic gene expression at this concentration, and some genes were found to be regulated down to the lowest concentration tested (1.6 µg/L) corresponding to approximately 1.5% of the human C(max) . Thus, at concentrations detected in European surface waters, diclofenac can affect the expression of multiple genes in exposed fish. Functional analysis of differentially expressed genes revealed effects on biological processes such as inflammation and immune response, in agreement with the mode of action of diclofenac in mammals. In contrast to some previously reported results, the bioconcentration factor was found to be stable (4.02 ± 0.75 for blood plasma and 2.54 ± 0.36 for liver) regardless of the water concentration. Environ. Toxicol. Chem. © 2011 SETAC.

sted, utgiver, år, opplag, sider
New York: Pergamon , 2011. Vol. 30, nr 9, s. 2126-2134
Emneord [en]
Gene expression, Bioconcentration, Diclofenac, Rainbow trout, Microarray
HSV kategori
Identifikatorer
URN: urn:nbn:se:umu:diva-45069DOI: 10.1002/etc.599PubMedID: 21688307OAI: oai:DiVA.org:umu-45069DiVA, id: diva2:428495
Tilgjengelig fra: 2011-06-30 Laget: 2011-06-21 Sist oppdatert: 2018-06-08bibliografisk kontrollert

Open Access i DiVA

Fulltekst mangler i DiVA

Andre lenker

Forlagets fulltekstPubMed

Personposter BETA

Fick, Jerker

Søk i DiVA

Av forfatter/redaktør
Fick, Jerker
Av organisasjonen
I samme tidsskrift
Environmental Toxicology and Chemistry

Søk utenfor DiVA

GoogleGoogle Scholar

doi
pubmed
urn-nbn

Altmetric

doi
pubmed
urn-nbn
Totalt: 195 treff
RefereraExporteraLink to record
Permanent link

Direct link
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annet format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annet språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf