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EFFECT OF VANADATE ON PHOTOSYNTHESIS AND THE ATP ADP RATIO IN LOW-CO2-ADAPTED CHLAMYDOMONAS-REINHARDTII CELLS
Umeå universitet, Teknisk-naturvetenskapliga fakulteten, Institutionen för fysiologisk botanik. Umeå universitet, Teknisk-naturvetenskapliga fakulteten, Umeå Plant Science Centre (UPSC).ORCID-id: 0000-0002-6002-929X
Umeå universitet, Teknisk-naturvetenskapliga fakulteten, Institutionen för fysiologisk botanik. Umeå universitet, Teknisk-naturvetenskapliga fakulteten, Umeå Plant Science Centre (UPSC).
Umeå universitet, Teknisk-naturvetenskapliga fakulteten, Institutionen för fysiologisk botanik. Umeå universitet, Teknisk-naturvetenskapliga fakulteten, Umeå Plant Science Centre (UPSC).ORCID-id: 0000-0001-5900-7395
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1994 (Engelska)Ingår i: Planta, ISSN 0032-0935, E-ISSN 1432-2048, Vol. 192, nr 1, s. 46-51Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

We have assessed the effect of vanadate as an inhibitor of plasma-membrane ATPase on photosynthesis and the ATP/ADP ratio in Chlamydomonas reinhardtii CW-92 (a mutant strain lacking a cell wall). This effect was compared in low-CO2-adapted cells grown in media bubbled with air containing 400 or 70 muL . L-1 CO2. Evidence is presented indicating that cells grown at 70 muL . L-1 CO2 have a higher rate of photosynthetic O2 evolution than cells grown at 400 muL . L-1 CO2, at limiting carbon concentrations. Extracellular and intracellular carbonic-anhydrase activities were, however, similar in cells grown in both of the low-carbon conditions. Vanadate inhibited, to a different extent, the HCO3--dependent O2 evolution in cells grown at 400 and 70 muL . L-1 CO2. At 400 muM vanadate, inhibition reached 70-75 % in cells grown at 400 muL . L-1 but only 50 % in those grown at 70 muL . L-1 CO2. The ATP/ADP ratios determined with and without vanadate at limiting concentrations of dissolved inorganic carbon indicated that more ATP was hydrolysed in algae grown at 70 muL . L-1 than in those grown at 400 muL . L-1 CO2. We conclude that the maximal capacity to accumulate dissolved inorganic carbon is inversely related to the CO2 concentration in the medium. Activation and - or synthesis of vanadate-sensitive ATPase may be the major explanation for the higher capacity for HCO3--dependent O2 evolution in cells grown under limited CO2 concentrations.

Ort, förlag, år, upplaga, sidor
1994. Vol. 192, nr 1, s. 46-51
Nyckelord [en]
ATPASE, CARBON CONCENTRATING MECHANISM, CARBONIC ANHYDRASE, CHLAMYDOMONAS, PHOTOSYNTHESIS
Identifikatorer
URN: urn:nbn:se:umu:diva-44649ISI: A1994MG36300006OAI: oai:DiVA.org:umu-44649DiVA, id: diva2:434127
Tillgänglig från: 2011-08-12 Skapad: 2011-06-09 Senast uppdaterad: 2018-06-08

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Karlsson, JanHiltonen, ThomasGardeström, PerSamuelsson, Göran

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