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Cleavage of IgG(1) and IgG(3) by gingipain K from Porphyromonas gingivalis may compromise host defense in progressive periodontitis
Umeå universitet, Medicinska fakulteten, Institutionen för molekylärbiologi (Medicinska fakulteten).
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2011 (engelsk)Inngår i: The FASEB Journal, ISSN 0892-6638, E-ISSN 1530-6860, Vol. 25, nr 10, s. 3741-3750Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

Degradation of immunoglobulins is an effective strategy of bacteria to evade the immune system. We have tested whether human IgG is a substrate for gingipain K of Porphyromonas gingivalis and found that the enzyme can hydrolyze subclass 1 and 3 of human IgG. The heavy chain of IgG(1) was cleaved at a single site within the hinge region, generating Fab and Fc fragments. IgG(3) was also cleaved within the heavy chain, but at several sites around the CH(2) region. Investigation of the enzyme kinetics of IgG proteolysis by gingipain K, using FPLC- and isothermal titration calorimetry-based assays followed by Hill plots, revealed non-Michaelis-Menten kinetics involving a mechanism of positive cooperativity. In ex vivo studies, it was shown that gingipain K retained its IgG hydrolyzing activity in human plasma despite the high content of natural protease inhibitors; that IgG(1) cleavage products were detected in gingival crevicular fluid samples from patients with severe periodontitis; and that gingipain K treatment of serum samples from patients with high antibody titers against P. gingivalis significantly hindered opsonin-dependent phagocytosis of clinical isolates of P. gingivalis by neutrophils. Altogether, these findings underline a biological function of gingipain K as an IgG protease of pathophysiological importance.-Vincents, B., Guentsch, A., Kostolowska, D., von Pawel-Rammingen, U., Eick, S., Potempa, J., Abrahamson, M. Cleavage of IgG(1) and IgG(3) by gingipain K from Porphyromonas gingivalis may compromise host defense in progressive periodontitis. FASEB J. 25, 3741-3750 (2011). www.fasebj.org

sted, utgiver, år, opplag, sider
Bethesda, Md.: Federation of American Societies for Experimental Biology , 2011. Vol. 25, nr 10, s. 3741-3750
Emneord [en]
cysteine proteases, cysteine peptidases, host-pathogen interactions, enzyme kinetics, immunoglobulin G, native substrates
HSV kategori
Identifikatorer
URN: urn:nbn:se:umu:diva-48446DOI: 10.1096/fj.11-187799ISI: 000295356400042OAI: oai:DiVA.org:umu-48446DiVA, id: diva2:450967
Tilgjengelig fra: 2011-10-24 Laget: 2011-10-20 Sist oppdatert: 2018-06-08bibliografisk kontrollert

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