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Use of genetically marked minicells as a probe in measurement of predation on bacteria in aquatic environments
Umeå universitet, Teknisk-naturvetenskapliga fakulteten, Institutionen för molekylärbiologi (Teknisk-naturvetenskaplig fakultet). (Johan Wikner)
Umeå universitet, Teknisk-naturvetenskapliga fakulteten, Institutionen för molekylärbiologi (Teknisk-naturvetenskaplig fakultet).
Umeå universitet, Teknisk-naturvetenskapliga fakulteten, Institutionen för molekylärbiologi (Teknisk-naturvetenskaplig fakultet).
1986 (engelsk)Inngår i: Applied and Environmental Microbiology, ISSN 0099-2240, E-ISSN 1098-5336, Vol. 52, nr 1, s. 4-8Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

Minicells produced by Escherichia coli M2141 were used as probes to measure predation on pelagic bacteria in situ. The minicells, labeled with [35S]methionine in one specific protein, were shown to disappear in the presence of a microflagellate (Ochromonas sp.), as seen by a decrease in the amount of labeled marker protein with time. Incubation in filtered (pore size, 0.2 μm) and autoclaved seawater did not affect the amount of labeled marker protein in the minicell. The generation time of flagellates feeding on minicells was determined to be similar to that found for flagellates grown on seawater bacteria or living E. coli NC3. Data indicate that minicells are seen as true food particles by the flagellates. The minicell probe was used in recapture experiments, in which predation in situ on pelagic bacteria was demonstrated. The rate of bacterial production showed a clear covariation with the rate of predation, both in different sea areas and in depth profiles. The obtained results (11 field experiments) showed that the rate of predation, on average, accounts for the consumption of 62% of the bacteria produced.

sted, utgiver, år, opplag, sider
American Society for Microbiology , 1986. Vol. 52, nr 1, s. 4-8
HSV kategori
Identifikatorer
URN: urn:nbn:se:umu:diva-52562ISI: A1986D078300002OAI: oai:DiVA.org:umu-52562DiVA, id: diva2:506372
Tilgjengelig fra: 2012-02-28 Laget: 2012-02-24 Sist oppdatert: 2018-06-08bibliografisk kontrollert
Inngår i avhandling
1. Predation on marine pelagic bacteria: On the in situ rates, regulation and dynamics of bacterivory studied with a molecular genetic technique
Åpne denne publikasjonen i ny fane eller vindu >>Predation on marine pelagic bacteria: On the in situ rates, regulation and dynamics of bacterivory studied with a molecular genetic technique
1989 (engelsk)Doktoravhandling, med artikler (Annet vitenskapelig)
sted, utgiver, år, opplag, sider
Umeå: Umeå universitet, 1989. s. 58
Emneord
Marine, grazing, minicells, molecular genetics, method, predation rate, dynamics, bacterivore, predator-prey
HSV kategori
Forskningsprogram
mikrobiologi
Identifikatorer
urn:nbn:se:umu:diva-52569 (URN)91-7174-418-5 (ISBN)
Disputas
1989-05-26, Institutionen för Mikrobiologi, Föreläsningssalen, Umeå Universitet 901 87, Umeå, 09:00 (engelsk)
Opponent
Veileder
Tilgjengelig fra: 2012-03-05 Laget: 2012-02-24 Sist oppdatert: 2018-06-08bibliografisk kontrollert

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