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Surface characterization of bone graft substitute materials conditioned in cell culture medium. 2. Protein adsorption
Umeå universitet, Teknisk-naturvetenskapliga fakulteten, Kemiska institutionen.
Umeå universitet, Medicinska fakulteten, Institutionen för odontologi, Ortodonti.
Umeå universitet, Medicinska fakulteten, Institutionen för odontologi, Ortodonti.
2012 (engelsk)Inngår i: Surface and Interface Analysis, ISSN 0142-2421, E-ISSN 1096-9918, Vol. 44, nr 8, s. 919-923Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

Three bone graft substitute materials (Bioglass 45S5, Bio-Oss (R) and Algipore (R)) were conditioned in a-minimum essential medium (alpha-MEM), with the addition of 10% fetal bovine serum (FBS), for 1 and 7?days. The chemistry of their solid-solution interface was monitored by X-ray photoelectron spectroscopy, using fast-frozen sample technique, and compared to that reported for original alpha-MEM. FBS added to the biological medium causes significant changes in the interface after only 1day of conditioning. Interfacial chemical composition and N 1s spectra show immediate adsorption of proteins at the surface of all three biomaterials, independent of their surface charge and chemical composition. However, the atomic ratio C/N and the C 1s spectra indicate a different orientation of adsorbed serum proteins, which is dependent on the particle's surface charge. Moreover, the adsorption of serum proteins at the surface of Bio-Oss causes a charge reversal at the interface, as evidenced by the change in the atomic ratio of Na/Cl. In addition to the particle's surface charge, the formation of the protein interfacial layer at the surface of the biomaterial seems to be the second major phenomenon important for subsequent cell recognition and the initiation of biomineralization. Copyright (c) 2012 John Wiley & Sons, Ltd.

sted, utgiver, år, opplag, sider
Hoboken, NJ: Wiley-Blackwell, 2012. Vol. 44, nr 8, s. 919-923
Emneord [en]
cryogenic XPS, solid-liquid interface, bone graft substitute, biomaterial, Bio-Oss, Algipore, Bioglass 45S5, serum, protein adsorption
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Identifikatorer
URN: urn:nbn:se:umu:diva-60088DOI: 10.1002/sia.5012ISI: 000306662600009OAI: oai:DiVA.org:umu-60088DiVA, id: diva2:558830
Tilgjengelig fra: 2012-10-05 Laget: 2012-10-01 Sist oppdatert: 2018-06-08bibliografisk kontrollert

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Shchukarev, AndreyMladenovic, ZivkoRansjö, Maria

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