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Cytokine profiles for human V gamma 9+ T cells stimulated by Plasmodium falciparum.
Umeå universitet, Medicinska fakulteten, Institutionen för klinisk mikrobiologi, Immunologi/immunkemi.
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1995 (Engelska)Ingår i: Parasite immunology (Print), ISSN 0141-9838, E-ISSN 1365-3024, Vol. 17, nr 8, s. 413-23Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

V gamma 9+ T cells from malaria non-exposed donors make proliferative responses to Plasmodium falciparum on in vitro stimulation. V gamma 9+ cells are strongly activated by components of the schizont stage of the parasite and by antigens released into the culture upon schizogony, while CD4+V gamma 9- cells are stimulated by the earlier stages of the parasite. Using reverse transcriptase-polymerase chain reaction (RT-PCR) we determined mRNA expression for 14 cytokines in highly purified V gamma 9+ cells enriched by positive selection after in vitro stimulation with P. falciparum schizont antigens. Interferon-gamma (IFN-gamma) and Tumor Necrosis Factor-alpha (TNF-alpha) were detected in all samples tested. The majority of samples also expressed TNF-beta, transforming growth factor-beta (TGF-beta) and Interleukin-8 (IL-8). Only occasional samples expressed IL-2, IL-5 and IL-10. Using the ELISPOT assay we found that a large fraction of the reactive V gamma 9+ cells produced IFN-gamma and that gamma delta T cells are the major producers of IFN-gamma in cultures stimulated with schizont antigens. The majority of V gamma 9+ cells in these cultures also express the membrane-bound form of TNF-alpha. Expression of these cytokines speaks for a cytolytic and/or inflammatory role of gamma delta cells in the response to malaria in non-exposed individuals.

Ort, förlag, år, upplaga, sidor
1995. Vol. 17, nr 8, s. 413-23
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Immunologi
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URN: urn:nbn:se:umu:diva-67899PubMedID: 7501422OAI: oai:DiVA.org:umu-67899DiVA, id: diva2:614792
Tillgänglig från: 2013-04-07 Skapad: 2013-04-07 Senast uppdaterad: 2018-06-08

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Hammarström, Marie-Louise

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