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Performance in omics analyses of blood samples in long-term storage: opportunities for the exploitation of existing biobanks in environmental health research
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2013 (Engelska)Ingår i: Journal of Environmental Health Perspectives, ISSN 0091-6765, E-ISSN 1552-9924, Vol. 121, nr 4, s. 480-487Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

Background: The suitability for omic analysis of biosamples collected in previous decades and currently stored in biobanks is unknown.

Objectives: We evaluated the influence of handling and storage conditions of blood-derived biosamples on transcriptomic, epigenomic (CpG methylation), plasma metabolomic [UPLC-ToFMS (ultra performance liquid chromatography-time-of-flight mass spectrometry)], and wide-target proteomic profiles.

Methods: We collected fresh blood samples without RNA preservative in heparin, EDTA, or citrate and held them at room temperature for ≤ 24 hr before fractionating them into buffy coat, erythrocytes, and plasma and freezing the fractions at -80oC or in liquid nitrogen. We developed methodology for isolating RNA from the buffy coats and conducted omic analyses. Finally, we analyzed analogous samples from the EPIC-Italy and Northern Sweden Health and Disease Study biobanks.

Results: Microarray-quality RNA could be isolated from buffy coats (including most biobank samples) that had been frozen within 8 hr of blood collection by thawing the samples in RNA preservative. Different anticoagulants influenced the metabolomic, proteomic, and to a lesser extent transcriptomic profiles. Transcriptomic profiles were most affected by the delay (as little as 2 hr) before blood fractionation, whereas storage temperature had minimal impact. Effects on metabolomic and proteomic profiles were noted in samples processed ≥ 8 hr after collection, but no effects were due to storage temperature. None of the variables examined significantly influenced the epigenomic profiles. No systematic influence of time-in-storage was observed in samples stored over a period of 13-17 years.

Conclusions: Most samples currently stored in biobanks are amenable to meaningful omics analysis, provided that they satisfy collection and storage criteria defined in this study.

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2013. Vol. 121, nr 4, s. 480-487
Nyckelord [en]
biomarkers, epigenomics, metabolomics, metabonomics, molecular epidemiology, proteomics, transcriptomics
Nationell ämneskategori
Arbetsmedicin och miljömedicin Folkhälsovetenskap, global hälsa, socialmedicin och epidemiologi Farmakologi och toxikologi
Identifikatorer
URN: urn:nbn:se:umu:diva-80868DOI: 10.1289/ehp.1205657ISI: 000323706100033PubMedID: 23384616OAI: oai:DiVA.org:umu-80868DiVA, id: diva2:651745
Tillgänglig från: 2013-09-27 Skapad: 2013-09-27 Senast uppdaterad: 2018-06-08Bibliografiskt granskad

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Bergdahl, Ingvar A.Hallmans, Göran

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Journal of Environmental Health Perspectives
Arbetsmedicin och miljömedicinFolkhälsovetenskap, global hälsa, socialmedicin och epidemiologiFarmakologi och toxikologi

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