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Peptidoglycan at its peaks: how chromatographic analyses can reveal bacterial cell wall structure and assembly
Department of Bioengineering, Stanford University, Stanford, CA, USA.
CBM ‘Severo Ochoa’ CSIC-UAM, Madrid, Spain.
Umeå universitet, Medicinska fakulteten, Molekylär Infektionsmedicin, Sverige (MIMS). Umeå universitet, Medicinska fakulteten, Institutionen för molekylärbiologi (Medicinska fakulteten). CBM ‘Severo Ochoa’ CSIC-UAM, Madrid, Spain.
Department of Bioengineering, Stanford University, Stanford, CA, USA ; Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, CA, USA.
2013 (Engelska)Ingår i: Molecular Microbiology, ISSN 0950-382X, E-ISSN 1365-2958, Vol. 89, nr 1, s. 1-13Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

The peptidoglycan (PG) cell wall is a unique macromolecule responsible for both shape determination and cellular integrity under osmotic stress in virtually all bacteria. A quantitative understanding of the relationships between PG architecture, morphogenesis, immune system activation and pathogenesis can provide molecular-scale insights into the function of proteins involved in cell wall synthesis and cell growth. High-performance liquid chromatography (HPLC) has played an important role in our understanding of the structural and chemical complexity of the cell wall by providing an analytical method to quantify differences in chemical composition. Here, we present a primer on the basic chemical features of wall structure that can be revealed through HPLC, along with a description of the applications of HPLC PG analyses for interpreting the effects of genetic and chemical perturbations to a variety of bacterial species in different environments. We describe the physical consequences of different PG compositions on cell shape, and review complementary experimental and computational methodologies for PG analysis. Finally, we present a partial list of future targets of development for HPLC and related techniques.

Ort, förlag, år, upplaga, sidor
2013. Vol. 89, nr 1, s. 1-13
Nationell ämneskategori
Medicinsk bioteknologi (med inriktning mot cellbiologi (inklusive stamcellsbiologi), molekylärbiologi, mikrobiologi, biokemi eller biofarmaci)
Identifikatorer
URN: urn:nbn:se:umu:diva-81856DOI: 10.1111/mmi.12266ISI: 000320728800001PubMedID: 23679048OAI: oai:DiVA.org:umu-81856DiVA, id: diva2:658599
Tillgänglig från: 2013-10-22 Skapad: 2013-10-22 Senast uppdaterad: 2018-06-08Bibliografiskt granskad

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Cava, Felipe

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Molekylär Infektionsmedicin, Sverige (MIMS)Institutionen för molekylärbiologi (Medicinska fakulteten)
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Molecular Microbiology
Medicinsk bioteknologi (med inriktning mot cellbiologi (inklusive stamcellsbiologi), molekylärbiologi, mikrobiologi, biokemi eller biofarmaci)

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