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Diagnostic biomarkers of prostate cancer
Karolinska Institutet, Stockholm.
Karolinska Institutet.
Karolinska Institutet, Stockholm.
Akademiska Hospital, Uppsala.
Vise andre og tillknytning
2011 (engelsk)Inngår i: Scandinavian Journal of Urology and Nephrology, ISSN 0036-5599, E-ISSN 1651-2065, Vol. 45, nr 1, s. 60-67Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

OBJECTIVE: Diagnostic tissue biomarkers for prostate cancer (PC) include basal cell markers and α-methylacyl-coenzyme A-racemase (AMACR), often used in combination. Their sensitivity and specificity are not perfect and there is a need for additional diagnostic biomarkers for PC in cases that are difficult to diagnose on routine stained sections.

MATERIAL AND METHODS: This study investigated the diagnostic accuracy of three novel tissue biomarkers for PC found through a search in the Human Protein Atlas database ( www.proteinatlas.com ): somatic cytochrome c (CYCS), intestinal cell kinase (ICK) and inhibitor of nuclear factor-κB kinase subunit beta (IKBKB), and compared the results with AMACR. A tissue microarray was constructed from 40 consecutive radical prostatectomy (RP) specimens including benign prostatic tissue, atrophy, high-grade prostatic intraepithelial neoplasia (HGPIN) and PC. Immunoreactivity was scored based on staining intensity and extent. Real-time polymerase chain reaction (PCR) was performed on malignant and benign frozen tissue samples from 32 RP specimens.

RESULTS: All four biomarkers showed a stronger expression in PC and HGPIN than in benign tissue (p < 0.001). The highest diagnostic accuracy for PC was achieved with ICK and AMACR at 97%. The area under the curve for CYCS, ICK, IKBKB and AMACR was 0.859, 0.997, 0.865 and 0.983, respectively. The presence of mRNA transcripts of the genes was confirmed by real-time PCR in benign and malignant prostatic tissue.

CONCLUSIONS: AMACR is an accurate diagnostic tissue marker for PC. However, in some PCs AMACR is false negative and a panel of CYCS, ICK and IKBKB may serve as ancillary diagnostic tool.

sted, utgiver, år, opplag, sider
Informa Healthcare, 2011. Vol. 45, nr 1, s. 60-67
Emneord [en]
AMACR, cytochrome c, ICK, IKBKB, immunohistochemistry, prostate, prostate cancer, tissue microarray
HSV kategori
Identifikatorer
URN: urn:nbn:se:umu:diva-82006DOI: 10.3109/00365599.2010.526141PubMedID: 21034352OAI: oai:DiVA.org:umu-82006DiVA, id: diva2:659309
Tilgjengelig fra: 2013-10-25 Laget: 2013-10-25 Sist oppdatert: 2018-06-08bibliografisk kontrollert

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