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Synthetic crowding agent dextran causes excluded volume interactions exclusively to tracer protein apoazurin
Umeå universitet, Teknisk-naturvetenskapliga fakulteten, Kemiska institutionen. (Pernilla Wittungs-Stafshede)
Umeå universitet, Teknisk-naturvetenskapliga fakulteten, Kemiska institutionen. (Biological Chemistry)
2014 (engelsk)Inngår i: FEBS Letters, ISSN 0014-5793, E-ISSN 1873-3468, Vol. 588, nr 5, s. 811-814Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

To understand protein biophysics in crowded cellular environments, researchers often use synthetic polymers as 'crowding agents' in vitro. The idea is that these agents will occupy space and reproduce the in vivo scenario in terms of excluded volume. However, recent work has challenged this concept and pointed out that attractive interactions between protein and crowding agent will provide an enthalpic contribution to the overall effect on protein thermodynamics. Here we use a typical synthetic crowding agent and a well-studied model protein to demonstrate in a window of 50 K that the presence of dextran 20 affects apoazurin by steric repulsion. (C) 2014 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

sted, utgiver, år, opplag, sider
Elsevier, 2014. Vol. 588, nr 5, s. 811-814
Emneord [en]
Macromolecular crowding, Crowding agent, Excluded volume, Attractive interaction, Spectroscopy, Azurin
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Identifikatorer
URN: urn:nbn:se:umu:diva-87389DOI: 10.1016/j.febslet.2014.01.043ISI: 000331717500026OAI: oai:DiVA.org:umu-87389DiVA, id: diva2:709338
Tilgjengelig fra: 2014-04-01 Laget: 2014-03-31 Sist oppdatert: 2018-06-08bibliografisk kontrollert

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