umu.sePublikationer
Ändra sökning
RefereraExporteraLänk till posten
Permanent länk

Direktlänk
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annat format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annat språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf
Development of an immunochromatography strip test based on truncated nucleocapsid antigens of three representative hantaviruses
Visa övriga samt affilieringar
2014 (Engelska)Ingår i: Virology Journal, ISSN 1743-422X, E-ISSN 1743-422X, Vol. 11, s. 87-Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

Background: Hantaviruses are causative agents of hemorrhagic fever with renal syndrome (HFRS) and nephropathia epidemica (NE) in the Old World and hantavirus pulmonary syndrome (HPS) in the New World. There is a need for time-saving diagnostic methods. In the present study, recombinant N antigens were used as antigens in an immunochromatography strip (ICG) test to detect specific IgG antibodies. Methods: The N-terminal 103 amino acids (aa) of Hantaan virus (HTNV), Puumala virus (PUUV) and Andes virus (ANDV) nucleocapsid (N) protein were expressed in E. coli as representative antigens of three groups (HFRS, NE and HPS-causing viruses) of hantavirus. Five different types of ICG test strips, one antigen line on one strip for each of the three selected hantaviruses (HTNV, PUUV and ANDV), three antigen lines on one strip and a mixed antigen line on one strip, were developed and sensitivities were compared. Results: A total of 87 convalescent-phase patient sera, including sera from 35 HFRS patients, 36 NE patients and 16 HPS patients, and 25 sera from healthy seronegative people as negative controls were used to evaluate the ICG test. Sensitivities of the three-line strip and mixed-line strip were similar to those of the single antigen strip (97.2 to 100%). On the other hand, all of the ICG test strips showed high specificities to healthy donors. Conclusion: These results indicated that the ICG test with the three representative antigens is an effective serodiagnostic tool for screening and typing of hantavirus infection in humans.

Ort, förlag, år, upplaga, sidor
2014. Vol. 11, s. 87-
Nyckelord [en]
Immunochromatography strip test, Hemorrhagic fever with renal syndrome (HFRS), Nephropathia epidemica (NE), Hantavirus pulmonary syndrome (HPS)
Nationell ämneskategori
Infektionsmedicin
Identifikatorer
URN: urn:nbn:se:umu:diva-91151DOI: 10.1186/1743-422X-11-87ISI: 000337307200001OAI: oai:DiVA.org:umu-91151DiVA, id: diva2:734364
Tillgänglig från: 2014-07-16 Skapad: 2014-07-15 Senast uppdaterad: 2018-06-07Bibliografiskt granskad

Open Access i DiVA

fulltext(738 kB)188 nedladdningar
Filinformation
Filnamn FULLTEXT01.pdfFilstorlek 738 kBChecksumma SHA-512
fdf5804f365423501d70bf66cbef506824c146adcc9d24f9774c89a6d9454bbd8fe70368de463ac4b1110de30efac559d0e2bd519cc13146ee8f6bfcbae3e33f
Typ fulltextMimetyp application/pdf

Övriga länkar

Förlagets fulltext

Personposter BETA

Ahlm, Clas

Sök vidare i DiVA

Av författaren/redaktören
Ahlm, Clas
Av organisationen
Infektionssjukdomar
I samma tidskrift
Virology Journal
Infektionsmedicin

Sök vidare utanför DiVA

GoogleGoogle Scholar
Totalt: 188 nedladdningar
Antalet nedladdningar är summan av nedladdningar för alla fulltexter. Det kan inkludera t.ex tidigare versioner som nu inte längre är tillgängliga.

doi
urn-nbn

Altmetricpoäng

doi
urn-nbn
Totalt: 716 träffar
RefereraExporteraLänk till posten
Permanent länk

Direktlänk
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annat format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annat språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf