umu.sePublikasjoner
Endre søk
RefereraExporteraLink to record
Permanent link

Direct link
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annet format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annet språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf
Immortalization of T-Cells Is Accompanied by Gradual Changes in CpG Methylation Resulting in a Profile Resembling a Subset of T-Cell Leukemias
Umeå universitet, Medicinska fakulteten, Institutionen för medicinsk biovetenskap, Patologi.
Umeå universitet, Teknisk-naturvetenskapliga fakulteten, Institutionen för matematik och matematisk statistik. Umeå universitet, Medicinska fakulteten, Institutionen för medicinsk biovetenskap, Patologi.
Vise andre og tillknytning
2014 (engelsk)Inngår i: Neoplasia, ISSN 1522-8002, E-ISSN 1476-5586, Vol. 16, nr 7, s. 606-615Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

We have previously described gene expression changes during spontaneous immortalization of T-cells, thereby identifying cellular processes important for cell growth crisis escape and unlimited proliferation. Here, we analyze the same model to investigate the role of genome-wide methylation in the immortalization process at different time points pre-crisis and post-crisis using high-resolution arrays. We show that over time in culture there is an overall accumulation of methylation alterations, with preferential increased methylation close to transcription start sites (TSSs), islands, and shore regions. Methylation and gene expression alterations did not correlate for the majority of genes, but for the fraction that correlated, gain of methylation close to TSS was associated with decreased gene expression. Interestingly, the pattern of CpG site methylation observed in immortal T-cell cultures was similar to clinical T-cell acute lymphoblastic leukemia (T-ALL) samples classified as CpG island methylator phenotype positive. These sites were highly overrepresented by polycomb target genes and involved in developmental, cell adhesion, and cell signaling processes. The presence of non-random methylation events in in vitro immortalized T-cell cultures and diagnostic T-ALL samples indicates altered methylation of CpG sites with a possible role in malignant hematopoiesis.

sted, utgiver, år, opplag, sider
2014. Vol. 16, nr 7, s. 606-615
HSV kategori
Identifikatorer
URN: urn:nbn:se:umu:diva-93242DOI: 10.1016/j.neo.2014.07.001ISI: 000340553900006OAI: oai:DiVA.org:umu-93242DiVA, id: diva2:774188
Forskningsfinansiär
Swedish Research Council, Dnr 340-2013-5185EU, FP7, Seventh Framework Programme, 200950Tilgjengelig fra: 2014-12-22 Laget: 2014-09-15 Sist oppdatert: 2018-06-07bibliografisk kontrollert

Open Access i DiVA

fulltext(2529 kB)245 nedlastinger
Filinformasjon
Fil FULLTEXT01.pdfFilstørrelse 2529 kBChecksum SHA-512
d01ecb7257b319923a5c0de74d0cd2ca215140e8de6ab5297c06cd19d40274cbd607da1832bdf6487ca6e671323b341b07644121956579b1db927e2aea64b9ed
Type fulltextMimetype application/pdf

Andre lenker

Forlagets fulltekst

Personposter BETA

Degerman, SofieLandfors, MattiasBorssen, MagnusEvelönn, EmmaForestier, ErikRyden, PatrikRoos, Göran

Søk i DiVA

Av forfatter/redaktør
Degerman, SofieLandfors, MattiasBorssen, MagnusEvelönn, EmmaForestier, ErikRyden, PatrikRoos, Göran
Av organisasjonen
I samme tidsskrift
Neoplasia

Søk utenfor DiVA

GoogleGoogle Scholar
Totalt: 245 nedlastinger
Antall nedlastinger er summen av alle nedlastinger av alle fulltekster. Det kan for eksempel være tidligere versjoner som er ikke lenger tilgjengelige

doi
urn-nbn

Altmetric

doi
urn-nbn
Totalt: 1256 treff
RefereraExporteraLink to record
Permanent link

Direct link
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annet format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annet språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf