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Crystal structure and functional characterization of Photosystem II-associated carbonic anhydrase CAH3 in Chlamydomonas reinhardtii
Department of Forest Genetics and Plant Physiology, SLU.
Umeå universitet, Teknisk-naturvetenskapliga fakulteten, Kemiska institutionen.
Umeå universitet, Teknisk-naturvetenskapliga fakulteten, Kemiska institutionen. Umeå universitet, Teknisk-naturvetenskapliga fakulteten, Institutionen för fysiologisk botanik. Umeå universitet, Teknisk-naturvetenskapliga fakulteten, Umeå Plant Science Centre (UPSC).
Umeå universitet, Teknisk-naturvetenskapliga fakulteten, Kemiska institutionen.
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2015 (Engelska)Ingår i: Plant Physiology, ISSN 0032-0889, E-ISSN 1532-2548, Vol. 167, nr 3, s. 950-962Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

In oxygenic photosynthesis, light energy is stored in the form of chemical energy by converting CO2 and water into carbohydrates.The light-driven oxidation of water that provides the electrons and protons for the subsequent CO2 fixation takes place inphotosystem II (PSII). Recent studies show that in higher plants, HCO3– increases PSII activity by acting as a mobile acceptor ofthe protons produced by PSII. In the green alga Chlamydomonas reinhardtii, a luminal carbonic anhydrase, CrCAH3, was suggested toimprove proton removal from PSII, possibly by rapid reformation of HCO3– from CO2. In this study, we investigated the interplaybetween PSII and CrCAH3 by membrane inlet mass spectrometry and x-ray crystallography. Membrane inlet mass spectrometrymeasurements showed that CrCAH3 was most active at the slightly acidic pH values prevalent in the thylakoid lumen underillumination. Two crystal structures of CrCAH3 in complex with either acetazolamide or phosphate ions were determined at 2.6- and2.7-Å resolution, respectively. CrCAH3 is a dimer at pH 4.1 that is stabilized by swapping of the N-terminal arms, a feature notpreviously observed in a-type carbonic anhydrases. The structure contains a disulfide bond, and redox titration of CrCAH3 functionwith dithiothreitol suggested a possible redox regulation of the enzyme. The stimulating effect of CrCAH3 and CO2/HCO3– on PSIIactivity was demonstrated by comparing the flash-induced oxygen evolution pattern of wild-type and CrCAH3-less PSIIpreparations. We showed that CrCAH3 has unique structural features that allow this enzyme to maximize PSII activity at lowpH and CO2 concentration.

Ort, förlag, år, upplaga, sidor
American Society of Plant Biologists , 2015. Vol. 167, nr 3, s. 950-962
Nationell ämneskategori
Botanik Biokemi och molekylärbiologi
Forskningsämne
biologisk kemi; biokemi
Identifikatorer
URN: urn:nbn:se:umu:diva-103651DOI: 10.1104/pp.114.253591ISI: 000354413900027OAI: oai:DiVA.org:umu-103651DiVA, id: diva2:814259
Forskningsfinansiär
Knut och Alice Wallenbergs StiftelseVetenskapsrådetTillgänglig från: 2015-05-26 Skapad: 2015-05-26 Senast uppdaterad: 2018-06-07Bibliografiskt granskad

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Shevela, DmitriyHainzl, TobiasGrundström, ChristinShutova, TatyanaMessinger, JohannesSamuelsson, GöranSauer-Eriksson, Elisabeth

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Shevela, DmitriyHainzl, TobiasGrundström, ChristinShutova, TatyanaMessinger, JohannesSamuelsson, GöranSauer-Eriksson, Elisabeth
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Kemiska institutionenInstitutionen för fysiologisk botanikUmeå Plant Science Centre (UPSC)
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Plant Physiology
BotanikBiokemi och molekylärbiologi

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