umu.sePublikasjoner
Endre søk
RefereraExporteraLink to record
Permanent link

Direct link
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annet format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annet språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf
The human gastrointestinal tract-specific transcriptome and proteome as defined by RNA sequencing and antibody-based profiling
Department of Immunology, Genetics and Pathology, Rudbeck Laboratory, Uppsala University.
Vise andre og tillknytning
2015 (engelsk)Inngår i: Journal of gastroenterology, ISSN 0944-1174, E-ISSN 1435-5922, Vol. 50, nr 1, s. 46-57Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

BACKGROUND: The gastrointestinal tract (GIT) is subdivided into different anatomical organs with many shared functions and characteristics, but also distinct differences. We have combined a genome-wide transcriptomics analysis with immunohistochemistry-based protein profiling to describe the gene and protein expression patterns that define the human GIT. METHODS: RNA sequencing data derived from stomach, duodenum, jejunum/ileum and colon specimens were compared to gene expression levels in 23 other normal human tissues analysed with the same method. Protein profiling based on immunohistochemistry and tissue microarrays was used to sub-localize the corresponding proteins with GIT-specific expression into sub-cellular compartments and cell types. RESULTS: Approximately 75% of all human protein-coding genes were expressed in at least one of the GIT tissues. Only 51 genes showed enriched expression in either one of the GIT tissues and an additional 83 genes were enriched in two or more GIT tissues. The list of GIT-enriched genes with validated protein expression patterns included various well-known but also previously uncharacterised or poorly studied genes. For instance, the colon-enriched expression of NXPE family member 1 (NXPE1) was established, while NLR family, pyrin domain-containing 6 (NLRP6) expression was primarily found in the human small intestine. CONCLUSIONS: We have applied a genome-wide analysis based on transcriptomics and antibody-based protein profiling to identify genes that are expressed in a specific manner within the human GIT. These genes and proteins constitute important starting points for an improved understanding of the normal function and the different states of disease associated with the GIT.

sted, utgiver, år, opplag, sider
Springer, 2015. Vol. 50, nr 1, s. 46-57
Emneord [en]
RNA expression, Immunohistochemistry, Gastrointestinal tract
HSV kategori
Identifikatorer
URN: urn:nbn:se:umu:diva-110913DOI: 10.1007/s00535-014-0958-7ISI: 000348982100006PubMedID: 24789573OAI: oai:DiVA.org:umu-110913DiVA, id: diva2:865878
Merknad

First online: 01 May 2014

Tilgjengelig fra: 2015-10-29 Laget: 2015-10-29 Sist oppdatert: 2018-06-07bibliografisk kontrollert

Open Access i DiVA

Fulltekst mangler i DiVA

Andre lenker

Forlagets fulltekstPubMed

Personposter BETA

Wanders, Alkwin

Søk i DiVA

Av forfatter/redaktør
Wanders, Alkwin
I samme tidsskrift
Journal of gastroenterology

Søk utenfor DiVA

GoogleGoogle Scholar

doi
pubmed
urn-nbn

Altmetric

doi
pubmed
urn-nbn
Totalt: 199 treff
RefereraExporteraLink to record
Permanent link

Direct link
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annet format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annet språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf