Umeå University's logo

umu.sePublikasjoner
Endre søk
RefereraExporteraLink to record
Permanent link

Direct link
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annet format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annet språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf
Theory for nonlinear dynamic force spectroscopy
Swedish Defence Research Agency (FOI), Umeå, Sweden.ORCID-id: 0000-0001-7719-944X
Umeå universitet, Teknisk-naturvetenskapliga fakulteten, Institutionen för fysik. Umeå universitet, Medicinska fakulteten, Umeå Centre for Microbial Research (UCMR). (The Biophysics and Biophotonics group)
2017 (engelsk)Inngår i: European Biophysics Journal, ISSN 0175-7571, E-ISSN 1432-1017, Vol. 46, nr 3, s. 225-233Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

Dynamic force spectroscopy (DFS) is an experimental technique that is commonly used to assess information on the strength, energy landscape, and lifetime of noncovalent bio-molecular interactions. DFS traditionally requires an applied force that increases linearly with time so that the bio-complex under investigation is exposed to a constant loading rate. However, tethers or polymers can modulate the applied force in a nonlinear manner. For example, bacterial adhesion pili and polymers with worm-like chain properties are structures that show nonlinear force responses. In these situations, the theory for traditional DFS cannot be readily applied. In this work, we expand the theory for DFS to also include nonlinear external forces while still maintaining compatibility with the linear DFS theory. To validate the theory, we modeled a bio- complex expressed on a stiff, an elastic, and a worm-like chain polymer, using Monte Carlo methods, and assessed the corresponding rupture force spectra. It was found that the nonlinear DFS (NLDFS) theory correctly predicted the numerical results. We also present a protocol suggesting an experimental approach and analysis method of the data to estimate the bond length and the thermal off-rate.

sted, utgiver, år, opplag, sider
2017. Vol. 46, nr 3, s. 225-233
Emneord [en]
AFM, Optical tweezers, Receptor, Ligand
HSV kategori
Identifikatorer
URN: urn:nbn:se:umu:diva-124645DOI: 10.1007/s00249-016-1158-6ISI: 000397496200002PubMedID: 27461369Scopus ID: 2-s2.0-84979686895OAI: oai:DiVA.org:umu-124645DiVA, id: diva2:953886
Forskningsfinansiär
Swedish Research Council, 2013-5379The Kempe FoundationsTilgjengelig fra: 2016-08-19 Laget: 2016-08-19 Sist oppdatert: 2023-03-24bibliografisk kontrollert

Open Access i DiVA

fulltext(1185 kB)213 nedlastinger
Filinformasjon
Fil FULLTEXT02.pdfFilstørrelse 1185 kBChecksum SHA-512
19bca5fc561a25d1fecc1ac324947677b36522b538320005ef9afe641ca36b94842e9678f5fcc524795b5eec04120d85aabf71f0113d50a4b260dd8ddf6aa325
Type fulltextMimetype application/pdf

Andre lenker

Forlagets fulltekstPubMedScopus

Person

Björnham, OscarAndersson, Magnus

Søk i DiVA

Av forfatter/redaktør
Björnham, OscarAndersson, Magnus
Av organisasjonen
I samme tidsskrift
European Biophysics Journal

Søk utenfor DiVA

GoogleGoogle Scholar
Totalt: 213 nedlastinger
Antall nedlastinger er summen av alle nedlastinger av alle fulltekster. Det kan for eksempel være tidligere versjoner som er ikke lenger tilgjengelige

doi
pubmed
urn-nbn

Altmetric

doi
pubmed
urn-nbn
Totalt: 757 treff
RefereraExporteraLink to record
Permanent link

Direct link
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annet format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annet språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf