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Activity-Based Proteome Profiling Probes Based on Woodward's Reagent K with Distinct Target Selectivity
Umeå University, Faculty of Science and Technology, Department of Chemistry.
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2016 (English)In: Angewandte Chemie International Edition, ISSN 1433-7851, E-ISSN 1521-3773, Vol. 55, no 27, 7766-7771 p.Article in journal (Refereed) Published
Abstract [en]

Woodward's reagent K (WRK) is a reactive heterocyclic compound that has been employed in protein chemistry to covalently and unspecifically label proteins at nucleophilic amino acids, notably at histidine and cysteine. We have developed a panel of WRK-derived activity-based probes and show that surprisingly and unexpectedly, these probes are fairly selective for a few proteins in the human proteome. The WRK-derived probes show unique reactivity towards the catalytic N-terminal proline in the macrophage migration inhibitory factor (MIF) and can be used to label and, if equipped with a fluorophore, to image MIF activities in living cells.

Place, publisher, year, edition, pages
2016. Vol. 55, no 27, 7766-7771 p.
National Category
Analytical Chemistry
Identifiers
URN: urn:nbn:se:umu:diva-126530DOI: 10.1002/anie.201602666ISI: 000383252900030PubMedID: 27159346OAI: oai:DiVA.org:umu-126530DiVA: diva2:1040020
Available from: 2016-10-26 Created: 2016-10-10 Last updated: 2016-10-26Bibliographically approved

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