umu.sePublications
Change search
ReferencesLink to record
Permanent link

Direct link
Fingerprinting the macro-organisation of pigment-protein complexes in plant thylakoid membranes in vivo by circular-dichroism spectroscopy
Show others and affiliations
2016 (English)In: Biochimica et Biophysica Acta - Bioenergetics, ISSN 0005-2728, E-ISSN 1879-2650, Vol. 1857, no 9, 1479-1489 p.Article in journal (Refereed) Published
Abstract [en]

Macro-organisation of the protein complexes in plant thylakoid membranes plays important roles in the regulation and fine-tuning of photosynthetic activity. These delicate structures might, however, undergo substantial changes during isolating the thylakoid membranes or during sample preparations, e.g., for electron microscopy. Circular-dichroism (CD) spectroscopy is a non-invasive technique which can thus be used on intact samples. Via excitonic and psi-type CD bands, respectively, it carries information on short-range excitonic pigment-pigment interactions and the macro-organisation (chiral macrodomains) of pigment-protein complexes (psi, polymer or salt-induced). In order to obtain more specific information on the origin of the major psi-type CD bands, at around (+)506, (-)674 and (+)690 nm, we fingerprinted detached leaves and isolated thylakoid membranes of wild-type and mutant plants and also tested the effects of different environmental conditions in vivo. We show that (i) the chiral macrodomains disassemble upon mild detergent treatments, but not after crosslinking the protein complexes; (ii) in different wild-type leaves of dicotyledonous and monocotyledonous angiosperms the CD features are quite robust, displaying very similar excitonic and psi-type bands, suggesting similar protein composition and (macro-) organisation of photosystem II (PSII) supercomplexes in the grana; (iii) the main positive psi-type bands depend on light-harvesting protein II contents of the membranes; (iv) the (+)506 nm band appears only in the presence of PSII-LHCII supercomplexes and does not depend on the xanthophyll composition of the membranes. Hence, CD spectroscopy can be used to detect different macro-domains in the thylakoid membranes with different outer antenna compositions in vivo. 

Place, publisher, year, edition, pages
2016. Vol. 1857, no 9, 1479-1489 p.
Keyword [en]
Circular dichroism, Chiral macrodomain, Light-harvesting complexes, Photosystem II supercomplexes, i-type CD, Thylakoid membrane, METER S, 1976, BIOCHEMICAL JOURNAL, V156, P469 Bianchi Silvia, 2011, PLANT CELL, V23, P2659
National Category
Biochemistry and Molecular Biology
Identifiers
URN: urn:nbn:se:umu:diva-126321DOI: 10.1016/j.bbabio.2016.04.287ISI: 000382590400014PubMedID: 27154055OAI: oai:DiVA.org:umu-126321DiVA: diva2:1044192
Available from: 2016-11-02 Created: 2016-10-03 Last updated: 2016-11-02Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full textPubMed

Search in DiVA

By author/editor
Schröder, Wolfgang P.
By organisation
Department of Chemistry
In the same journal
Biochimica et Biophysica Acta - Bioenergetics
Biochemistry and Molecular Biology

Search outside of DiVA

GoogleGoogle Scholar

Altmetric score

Total: 19 hits
ReferencesLink to record
Permanent link

Direct link