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Drosophila melanogaster transcriptional response to Nora virus infection
Umeå University, Faculty of Medicine, Department of Molecular Biology (Faculty of Medicine). (Dan Hultmark)
Umeå University, Faculty of Medicine, Department of Molecular Biology (Faculty of Medicine).
Umeå University, Faculty of Medicine, Department of Molecular Biology (Faculty of Medicine).
(English)Manuscript (preprint) (Other academic)
National Category
Cell and Molecular Biology
Identifiers
URN: urn:nbn:se:umu:diva-127350OAI: oai:DiVA.org:umu-127350DiVA: diva2:1045375
Available from: 2016-11-09 Created: 2016-11-09 Last updated: 2016-11-09
In thesis
1. Biology of a small RNA virus that infects Drosophila melanogaster
Open this publication in new window or tab >>Biology of a small RNA virus that infects Drosophila melanogaster
2016 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Drosophila melanogaster has been extensively used as a model organism to study diverse facets of biology, including host-pathogen interactions and the basic biology of its pathogens. I have used the fruit fly as a model to study elementary aspects of Nora virus biology, such as the role of the different proteins encoded by the virus genome. Nora virus, an enteric virus transmitted via the feca-oral route, does not cause any obvious pathology in the fly, although the infection is persistent. Nora virus genome consists of a positive strand RNA that is translated in four open reading frames (ORF).  Since sequence homology studies did not yield much information about the different Nora virus proteins, I have used the cDNA clone of the virus to construct mutants to identify the specific function of each protein. My results have shown that,

1) The protein(s) encoded by ORF 1 are crucial for the replication of the virus genome.

2) The C-terminus of the ORF 1-encoded protein (VP1), is an inhibitor to the RNAi pathway.

3) The transmembrane domain in the N-terminus of the ORF2-encoded protein (VP2) is important for the formation of Nora virus virions.

4) The ORF 3-encoded protein (VP3) forms α-helical trimers and this protein is essential for the stability of Nora virus capsid.                                                    

I have also performed RNA sequencing to investigate the transcriptional response of D. melanogaster in response to Nora virus infection and my results indicate that,                       

5) The upregulation of genes related to cellular stress and protein synthesis and the downregulation of basal digestive machinery, together with the induction of upd3, implies major gut epithelium damage and subsequent regeneration.

Place, publisher, year, edition, pages
Umeå: Umeå University, 2016. 52 p.
Keyword
Nora virus, Drosophila melanogaster, virus biology, host-pathogen interaction
National Category
Cell and Molecular Biology
Research subject
Molecular Biology
Identifiers
urn:nbn:se:umu:diva-127352 (URN)978-91-7601-593-3 (ISBN)
Public defence
2016-12-01, Hörsal 135, Byggnad 9A, Norrlands Universitetssjukhus, Umeå, 14:00 (English)
Opponent
Supervisors
Available from: 2016-11-10 Created: 2016-11-09 Last updated: 2016-11-24Bibliographically approved

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Sadanandan, Sajna AnandEkström, Jens-OlaHultmark, Dan
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