Transcriptomic and phenotypic analysis reveals new functions for the Tat pathway in Yersinia pseudotuberculosis
2016 (English)In: Journal of Bacteriology, ISSN 0021-9193, E-ISSN 1098-5530, Vol. 198, no 20, 2876-2886 p.Article in journal (Refereed) Published
The Twin-arginine translocation (Tat) system mediates secretion of folded proteins that in bacteria, plants and archaea are identified via an N-terminal signal peptide. Tat systems are associated with virulence in many bacterial pathogens and our previous studies revealed that Tat deficient Yersinia pseudotuberculosis was severely attenuated for virulence. Aiming to identify Tat-dependent pathways and phenotypes of relevance for in vivo infection, we analysed the global transcriptome of parental and ∆tatC mutant strains of Y. pseudotuberculosis during exponential and stationary growth at 26oC and 37oC. The most significant changes in the transcriptome of the ∆tatC mutant were seen at 26oC during stationary phase growth and these included the altered expression of genes related to virulence, stress responses and metabolism. Subsequent phenotypic analysis based on these transcriptome changes revealed several novel Tat-dependent phenotypes including decreased YadA expression, impaired growth under iron-limiting and high copper conditions as well as acidic pH and SDS. Several functionally related Tat substrates were also verified to contribute to these phenotypes. Interestingly, the phenotypic defects observed in the Tat-deficient strain were generally more pronounced than in mutants lacking the Tat substrate predicted to contribute to that specific function. Altogether, this provides new insight into the impact of Tat deficiency on in vivo fitness and survival/replication of Y. pseudotuberculosis during infection.
Place, publisher, year, edition, pages
Washington: American Society for Microbiology , 2016. Vol. 198, no 20, 2876-2886 p.
Yersinia pseudotuberculosis, Tat pathway, virulence, stress response, transcriptome analysis
Microbiology Bioinformatics and Systems Biology Biochemistry and Molecular Biology
Research subject Infectious Diseases; Microbiology; Molecular Biology
IdentifiersURN: urn:nbn:se:umu:diva-128029DOI: 10.1128/JB.00352-16ISI: 000384347500014OAI: oai:DiVA.org:umu-128029DiVA: diva2:1048911