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Method for accurate fiber length determination from increment cores for large-scale population analyses in Norway spruce
Umeå universitet, Teknisk-naturvetenskapliga fakulteten, Institutionen för matematik och matematisk statistik.
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2016 (Engelska)Ingår i: Holzforschung, ISSN 0018-3830, E-ISSN 1437-434X, Vol. 70, nr 9, s. 829-838Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

Fiber (tracheid) length is an important trait targeted for genetic and silvicultural improvement. Such studies require large-scale non-destructive sampling, and accurate length determination. The standard procedure for non-destructive sampling is to collect increment cores, singularize their cells by maceration, measure them with optical analyzer and apply various corrections to suppress influence of non-fiber particles and cut fibers, as fibers are cut by the corer. The recently developed expectation-maximization method (EM) not only addresses the problem of non-fibers and cut fibers, but also corrects for the sampling bias. Here, the performance of the EM method has been evaluated by comparing it with length-weighing and squared length-weighing, both implemented in fiber analyzers, and with microscopy data for intact fibers, corrected for sampling bias, as the reference. This was done for 12-mm increment cores from 16 Norway spruce (Picea abies (L.) Karst) trees on fibers from rings 8-11 (counted from pith), representing juvenile wood of interest in breeding programs. The EM-estimates provided mean-fiber-lengths with bias of only +2.7% and low scatter. Length-weighing and length2-weighing gave biases of -7.3% and +9.3%, respectively, and larger scatter. The suggested EM approach constitutes a more accurate non-destructive method for fiber length (FL) determination, expected to be applicable also to other conifers.

Ort, förlag, år, upplaga, sidor
Walter de Gruyter, 2016. Vol. 70, nr 9, s. 829-838
Nyckelord [en]
expectation-maximization, fiber length, increment core, optical fiber analyzer, Picea abies, tracheid length
Nationell ämneskategori
Trävetenskap Pappers-, massa- och fiberteknik
Identifikatorer
URN: urn:nbn:se:umu:diva-127630DOI: 10.1515/hf-2015-0138ISI: 000385808000004OAI: oai:DiVA.org:umu-127630DiVA, id: diva2:1050787
Projekt
Bio4EnergyTillgänglig från: 2016-11-30 Skapad: 2016-11-16 Senast uppdaterad: 2019-09-06Bibliografiskt granskad

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Abramowicz, KonradSjöstedt de Luna, Sara

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Abramowicz, KonradSjöstedt de Luna, Sara
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Institutionen för matematik och matematisk statistik
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Holzforschung
TrävetenskapPappers-, massa- och fiberteknik

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