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Multicenter validation of CSF neurofilaments as diagnostic biomarkers for ALS
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2016 (English)In: Amyotrophic Lateral Sclerosis and Frontotemporal Degeneration, ISSN 2167-8421, E-ISSN 2167-9223, Vol. 17, no 5-6, 404-413 p.Article in journal (Refereed) Published
Abstract [en]

OBJECTIVE: Neurofilaments are leading neurochemical biomarkers for amyotrophic lateral sclerosis (ALS). Here, we investigated the effect of preanalytical factors on neurofilament concentrations in cerebrospinal fluid (CSF) in a reverse round-robin with 15 centers across Europe/U.S. METHODS: Samples from ALS and control patients (5/5 each center, n=150) were analyzed for phosphorylated neurofilament heavy chain (pNfH) and neurofilament light chain (NfL) at two laboratories. RESULTS: CSF pNfH was increased (p<0.05) in ALS in 10 out of 15 centers and NfL in 5 out of 12 centers. The coefficient of variation (CV%) of pNfH measurements between laboratories was 18.7 +/- 19.1%. We calculated a diagnostic cut-off of >568.5pg/mL for pNfH (sensitivity 78.7%, specificity 93.3%) and >1,431pg/mL for NfL (sensitivity 79.0%, specificity 86.4%). CONCLUSION: Values in ALS patients are already comparable between most centers, supporting eventual implementation into clinical routine. However, continuous quality control programs will be necessary for inclusion in the diagnostic work-up.

Place, publisher, year, edition, pages
2016. Vol. 17, no 5-6, 404-413 p.
Keyword [en]
Cerebrospinal fluid, neurofilament, biomarker, amyotrophic lateral sclerosis, round robin
National Category
Rheumatology and Autoimmunity
Identifiers
URN: urn:nbn:se:umu:diva-127982DOI: 10.3109/21678421.2016.1167913ISI: 000381024500014PubMedID: 27415180OAI: oai:DiVA.org:umu-127982DiVA: diva2:1051961
Available from: 2016-12-05 Created: 2016-11-21 Last updated: 2017-05-11Bibliographically approved

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Andersen, Peter M.Rikardsson, Anna-KarinOtto, Markus
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CiteExportLink to record
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Citation style
  • apa
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  • Other style
More styles
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  • de-DE
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More languages
Output format
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