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Effect of Aggregatibacter actinomycetemcomitans Leukotoxin on ATP Release through Pannexin Channels in Human Monocytes
Umeå University, Faculty of Medicine, Department of Odontology.
Umeå University, Faculty of Medicine, Department of Odontology.
2016 (English)Independent thesis Advanced level (professional degree), 20 credits / 30 HE creditsStudent thesis
Abstract [en]

Aggregatibacter actinomycetemcomitans is strongly associated with aggressive periodontitis and one of several virulence factors is a leukotoxin (LtxA). The toxin has a consequential impact on human leukocytes which leads to an interference with the host ́s defences due to a chain reaction involving activation and release of a pro-inflammatory cytokine; interleukin-1β (IL-1β). As an early phase in this reaction chain the toxin stimulates a massive release of adenosine triphosphate (ATP) from human leukocytes. We hypothesize that leukotoxin-induced pro- inflammatory cell death is initiated by ATP release through pannexin channels in the human monocyte cell membrane. The aim of this study was to investigate if blocking of pannexin channels with carbenoxolone (Cbx) results in a reduction of ATP release. A human monocyte cell line (THP-1 cells) was exposed to purified LtxA, Cbx and oxidized ATP. Colorimetric ATP kit was used to evaluate levels of ATP release and thereafter the samples were read in a spectrophotometer. This study confirms that LtxA induces an ATP release from THP-1 cells. Our conclusion is that blocking of pannexin channels does not result in a statistically significant reduction in ATP release, which indicates that ATP is released by one or several other undetected pathways. 

Place, publisher, year, edition, pages
2016. , 17 p.
Keyword [en]
LtxA, ATP release, Aggregatibacter actinomycetemcomitans
National Category
Medical and Health Sciences
Identifiers
URN: urn:nbn:se:umu:diva-128149OAI: oai:DiVA.org:umu-128149DiVA: diva2:1056891
Educational program
Dentistry Programme
Supervisors
Examiners
Available from: 2016-12-16 Created: 2016-11-24 Last updated: 2016-12-16Bibliographically approved

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CiteExportLink to record
Permanent link

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Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
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  • nn-NO
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More languages
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  • html
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