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Structural basis for glutathione-mediated activation of the virulence regulatory protein PrfA in Listeria
Umeå University, Faculty of Science and Technology, Department of Chemistry. Umeå University, Faculty of Medicine, Umeå Centre for Microbial Research (UCMR).
Umeå University, Faculty of Science and Technology, Department of Chemistry. Umeå University, Faculty of Medicine, Umeå Centre for Microbial Research (UCMR).
Umeå University, Faculty of Science and Technology, Department of Chemistry. Umeå University, Faculty of Medicine, Umeå Centre for Microbial Research (UCMR).
Umeå University, Faculty of Science and Technology, Department of Chemistry. Umeå University, Faculty of Medicine, Umeå Centre for Microbial Research (UCMR).
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2016 (English)In: Proceedings of the National Academy of Sciences of the United States of America, ISSN 0027-8424, E-ISSN 1091-6490, Vol. 113, no 51, p. 14733-14738Article in journal (Refereed) Published
Abstract [en]

Infection by the human bacterial pathogen Listeria monocytogenes is mainly controlled by the positive regulatory factor A (PrfA), a member of the Crp/Fnr family of transcriptional activators. Published data suggest that PrfA requires the binding of a cofactor for full activity, and it was recently proposed that glutathione (GSH) could fulfill this function. Here we report the crystal structures of PrfA in complex with GSH and in complex with GSH and its cognate DNA, the hly operator PrfA box motif. These structures reveal the structural basis for a GSH-mediated allosteric mode of activation of PrfA in the cytosol of the host cell. The crystal structure of PrfAWT in complex only with DNA confirms that PrfAWT can adopt a DNA binding-compatible structure without binding the GSH activator molecule. By binding to PrfA in the cytosol of the host cell, GSH induces the correct fold of the HTH motifs, thus priming the PrfA protein for DNA interaction.

Place, publisher, year, edition, pages
2016. Vol. 113, no 51, p. 14733-14738
Keywords [en]
Listeria, PrfA, activation, glutathione, virulence
National Category
Organic Chemistry Medical Genetics
Identifiers
URN: urn:nbn:se:umu:diva-128915DOI: 10.1073/pnas.1614028114ISI: 000390044900062OAI: oai:DiVA.org:umu-128915DiVA, id: diva2:1057771
Available from: 2016-12-19 Created: 2016-12-19 Last updated: 2018-06-09Bibliographically approved

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Hall, MichaelGrundström, ChristinBegum, AfshanLindberg, Mikael J.Sauer, Uwe H.Almqvist, FredrikJohansson, JörgenSauer-Eriksson, A. Elisabeth

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Hall, MichaelGrundström, ChristinBegum, AfshanLindberg, Mikael J.Sauer, Uwe H.Almqvist, FredrikJohansson, JörgenSauer-Eriksson, A. Elisabeth
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Department of ChemistryUmeå Centre for Microbial Research (UCMR)Department of Molecular Biology (Faculty of Medicine)Molecular Infection Medicine Sweden (MIMS)
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Proceedings of the National Academy of Sciences of the United States of America
Organic ChemistryMedical Genetics

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