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Detection of low levels of Escherichia coli by electrochemical impedance spectroscopy and singular value decomposition
Umeå University, Faculty of Science and Technology, Department of Chemistry.
2017 (English)In: Chemometrics and Intelligent Laboratory Systems, ISSN 0169-7439, E-ISSN 1873-3239, Vol. 163, 49-54 p.Article in journal (Refereed) Published
Abstract [en]

The first steps are reported in the development of a new ultrasensitive electrochemical biosensor for detection of Escherichia coli (E. coli) in water. Two gold electrodes in a sandwich flow-cell were modified with E. coli polyclonal antibody and exposed to three different concentrations of E. coli. Electrochemical Impedance Spectroscopy was used in combination with Singular Value Decompostion of the complex numbers to monitor the interactions at the electrode surfaces. A linear regression line in the concentration range 10-1000 CFU⁎ml-1 was obtained without use of redox probes or metal nanoparticles for signal amplification.

Place, publisher, year, edition, pages
Elsevier, 2017. Vol. 163, 49-54 p.
National Category
Analytical Chemistry
Identifiers
URN: urn:nbn:se:umu:diva-128935DOI: 10.1016/j.chemolab.2016.11.008ISI: 000399857600007OAI: oai:DiVA.org:umu-128935DiVA: diva2:1058050
Available from: 2016-12-20 Created: 2016-12-20 Last updated: 2017-05-29Bibliographically approved

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