umu.sePublications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Effects of Long-Term Storage Time and Original Sampling Month on Biobank Plasma Protein Concentrations
Umeå University, Faculty of Medicine, Department of Public Health and Clinical Medicine, Nutritional Research.
Umeå University, Faculty of Medicine, Department of Medical Biosciences, Clinical chemistry.
2016 (English)In: EBioMedicine, ISSN 0360-0637, E-ISSN 2352-3964, Vol. 12, 309-314 p.Article in journal (Refereed) Published
Abstract [en]

The quality of clinical biobank samples is crucial to their value for life sciences research. A number of factors related to the collection and storage of samples may affect the biomolecular composition. We have studied the effect of long-time freezer storage, chronological age at sampling, season and month of the year and on the abundance levels of 108 proteins in 380 plasma samples collected from 106 Swedish women. Storage time affected 18 proteins and explained 4.8–34.9% of the observed variance. Chronological age at sample collection after adjustment for storage-time affected 70 proteins and explained 1.1–33.5% of the variance. Seasonal variation had an effect on 15 proteins and month (number of sun hours) affected 36 proteins and explained up to 4.5% of the variance after adjustment for storage-time and age. The results show that freezer storage time and collection date (month and season) exerted similar effect sizes as age on the protein abundance levels. This implies that information on the sample handling history, in particular storage time, should be regarded as equally prominent covariates as age or gender and need to be included in epidemiological studies involving protein levels.

Place, publisher, year, edition, pages
2016. Vol. 12, 309-314 p.
Keyword [en]
Plasma proteins, Biobank, Covariate, Storage time, Sampling month, Proximity extension assay
National Category
Other Medical Biotechnology Other Medical Sciences not elsewhere specified
Identifiers
URN: urn:nbn:se:umu:diva-129414DOI: 10.1016/j.ebiom.2016.08.038ISI: 000386878500048PubMedID: 27596149OAI: oai:DiVA.org:umu-129414DiVA: diva2:1060041
Available from: 2016-12-27 Created: 2016-12-27 Last updated: 2017-01-25Bibliographically approved

Open Access in DiVA

fulltext(1401 kB)8 downloads
File information
File name FULLTEXT01.pdfFile size 1401 kBChecksum SHA-512
ee01c93e147fe78e0080b5a36890c8a4b55d2c1374bad09679244d6038159efeae93691e2fa810b7c3f1de2bff8124514787f8d945c2d45140834f5181de8e7e
Type fulltextMimetype application/pdf

Other links

Publisher's full textPubMed

Search in DiVA

By author/editor
Hallmans, GöranGrankvist, Kjell
By organisation
Nutritional ResearchClinical chemistry
In the same journal
EBioMedicine
Other Medical BiotechnologyOther Medical Sciences not elsewhere specified

Search outside of DiVA

GoogleGoogle Scholar
Total: 8 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 155 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf