umu.sePublications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Enhanced resistance of PsbS-deficient rice (Oryza sativa L.) to fungal and bacterial pathogens
Show others and affiliations
2016 (English)In: Journal of Plant Biology, ISSN 1226-9239, Vol. 59, no 6, 616-626 p.Article in journal (Refereed) Published
Abstract [en]

The 22-kDa PsbS protein of Photosystem II is involved in nonphotochemical quenching (NPQ) of chlorophyll fluorescence. Genome-wide analysis of the expression pattern in PsbS knockout (KO) rice plants showed that a lack of this protein led to changes in the transcript levels of 406 genes, presumably a result of superoxide produced in the chloroplasts. The top Gene Ontology categories, in which expression was the most differential, included 'Immune response', 'Response to jasmonic acid', and 'MAPK cascade'. From those genes, we randomly selected nine that were up-regulated. Our microarray results were confirmed by quantitative RT-PCR analysis. The KO and PsbS RNAi (knockdown) plants were more resistant to pathogens Magnaporthe oryzae PO6-6 and Xanthomonas oryzae pv. oryzae than either the wild-type plants or PsbS-overexpressing transgenic line. These findings suggest that superoxide production might be the reason that these plants have greater pathogen resistance to fungal and bacterial pathogens in the absence of energy-dependent NPQ. For example, a high level of cell wall lignification in the KO mutants was possibly due to enhanced superoxide production. Our data indicate that certain abiotic stress-induced reactive oxygen species can promote specific signaling pathways, which then activate a defense mechanism against biotic stress in PsbS-KO rice plants.

Place, publisher, year, edition, pages
Springer Berlin/Heidelberg, 2016. Vol. 59, no 6, 616-626 p.
Keyword [en]
Biotic stress tolerance, Microarray, Nonphotochemical quenching, PsbS, Rice, Superoxide
National Category
Botany Genetics
Identifiers
URN: urn:nbn:se:umu:diva-129724DOI: 10.1007/s12374-016-0068-6ISI: 000389986200006OAI: oai:DiVA.org:umu-129724DiVA: diva2:1063569
Available from: 2017-01-10 Created: 2017-01-09 Last updated: 2017-01-10Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full text

Search in DiVA

By author/editor
Hall, MichaelSkogström, OscarJansson, Stefan
By organisation
Umeå Plant Science Centre (UPSC)Department of Plant Physiology
In the same journal
Journal of Plant Biology
BotanyGenetics

Search outside of DiVA

GoogleGoogle Scholar

Altmetric score

Total: 17 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf