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The Dimeric Architecture of Checkpoint Kinases Mec1(ATR) and Tel1(ATM) Reveal a Common Structural Organization
Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, St. Louis, Missouri.
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2016 (Engelska)Ingår i: Journal of Biological Chemistry, ISSN 0021-9258, E-ISSN 1083-351X, Vol. 291, nr 26, s. 13436-13447Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

The phosphatidylinositol 3-kinase-related protein kinases are key regulators controlling a wide range of cellular events. The yeast Tel1 and Mec1Ddc2 complex (ATM and ATR-ATRIP in humans) play pivotal roles in DNA replication, DNA damage signaling, and repair. Here, we present the first structural insight for dimers of Mec1Ddc2 and Tel1 using single-particle electron microscopy. Both kinases reveal a head to head dimer with one major dimeric interface through the N-terminal HEAT (named after Huntingtin, elongation factor 3, protein phosphatase 2A, and yeast kinase TOR1) repeat. Their dimeric interface is significantly distinct from the interface of mTOR complex 1 dimer, which oligomerizes through two spatially separate interfaces. We also observe different structural organizations of kinase domains of Mec1 and Tel1. The kinase domains in the Mec1Ddc2 dimer are located in close proximity to each other. However, in the Tel1 dimer they are fully separated, providing potential access of substrates to this kinase, even in its dimeric form.

Ort, förlag, år, upplaga, sidor
2016. Vol. 291, nr 26, s. 13436-13447
Nyckelord [en]
checkpoint control, DNA damage response, nucleic acid enzymology, phosphatidylinositol kinase (PI Kinase), protein structure, serine, threonine protein kinase
Nationell ämneskategori
Medicinsk bioteknologi (med inriktning mot cellbiologi (inklusive stamcellsbiologi), molekylärbiologi, mikrobiologi, biokemi eller biofarmaci)
Identifikatorer
URN: urn:nbn:se:umu:diva-130036DOI: 10.1074/jbc.M115.708263ISI: 000379771100004PubMedID: 27129217OAI: oai:DiVA.org:umu-130036DiVA, id: diva2:1063933
Tillgänglig från: 2017-01-11 Skapad: 2017-01-11 Senast uppdaterad: 2018-06-09Bibliografiskt granskad

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Wanrooij, Paulina H.Darbari, Vidya C.

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Wanrooij, Paulina H.Darbari, Vidya C.
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Journal of Biological Chemistry
Medicinsk bioteknologi (med inriktning mot cellbiologi (inklusive stamcellsbiologi), molekylärbiologi, mikrobiologi, biokemi eller biofarmaci)

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