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Interleukin-6 reduces paraoxonase-1 activity in a dose-dependent manner: evidence for a potential novel lipoprotein-based modulatory mechanism
Umeå University, Faculty of Medicine, Department of Public Health and Clinical Medicine.
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2016 (English)In: Atherosclerosis, ISSN 0021-9150, E-ISSN 1879-1484, Vol. 252, p. E113-E114Article in journal, Meeting abstract (Other academic) Published
Abstract [en]

Objectives: The anti-oxidant/anti-inflammatory nature of HDL is mainly associated with paraoxonase-1 (PON1). Previous studies have revealed an inverse correlation between Interleukin-6 (IL-6) and PON1 expression. The current study investigates the effect of IL-6 on serum PON1 activity in vitro, given the potential structural capability of PON1 to host multiple ligands. Methods: PON1 activity was measured spectrophotometrically (234 nm) using paraoxon substrate in the presence of concentrations of IL-6 than control samples. A sequence alignment using the FASTA sequence was manually conducted to identify possible homologies between PON1 and the IL-6-binding protein. Statistical analysis was conducted using GraphPad Prism v5.0. Results: PON1 enzyme activity decreased by 15%, 26% (P<0.05) and 55% (P<0.001) in the presence of 4, 10 and 20 pg/ml of IL-6, respectively. in comparison with the controls. Student t. test was used as statistical method (p<0.05: statistically significant). There are potential homologies between PON1 active sites and know IL-6-binding residues. Conclusions: This study shows that IL-6 directly reduce the PON1 activity in a dose-dependent manner. This observation supports some studies indicating inverse correlation between PON1 and IL-6. However, as opposed to the gene-mediated approach, this study suggest that IL-6 may act directly through specific binding to PON1 (biochemical modulation). X ray crystallography can further scrutinize the present finding.

Place, publisher, year, edition, pages
Elsevier, 2016. Vol. 252, p. E113-E114
National Category
Cardiac and Cardiovascular Systems
Identifiers
URN: urn:nbn:se:umu:diva-130114DOI: 10.1016/j.atherosclerosis.2016.07.616ISI: 000388978400384OAI: oai:DiVA.org:umu-130114DiVA, id: diva2:1065102
Conference
Congress of the European-Atherosclerosis-Society (EAS), MAY 29-JUN 01, 2016, Innsbruck, AUSTRIA
Note

Meeting Abstract: EAS16-1009, Lipoproteins and Lipid Metabolism: HDL

Available from: 2017-01-13 Created: 2017-01-11 Last updated: 2018-06-09Bibliographically approved

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Abedpour Dehkordi, Adel

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