umu.sePublications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Colonization of patients, healthcare workers, and the environment with healthcare-associated Staphylococcus epidermidis genotypes in an intensive care unit: a prospective observational cohort study
Umeå University, Faculty of Medicine, Department of Clinical Microbiology.ORCID iD: 0000-0002-1483-4255
Umeå University, Faculty of Medicine, Department of Clinical Microbiology, Infectious Diseases.
Umeå University, Faculty of Medicine, Department of Clinical Microbiology.
Umeå University, Faculty of Medicine, Department of Clinical Microbiology.
Show others and affiliations
2016 (English)In: BMC Infectious Diseases, ISSN 1471-2334, E-ISSN 1471-2334, Vol. 16, 743Article in journal (Refereed) Published
Abstract [en]

Background: During the last decades, healthcare-associated genotypes of methicillin-resistant Staphylococcus epidermidis (HA-MRSE) have been established as important opportunistic pathogens. However, data on potential reservoirs on HA-MRSE is limited. The aim of the present study was to investigate the dynamics and to which extent HA-MRSE genotypes colonize patients, healthcare workers (HCWs) and the environment in an intensive care unit (ICU).

Methods: Over 12 months in 2006-2007, swab samples were obtained from patients admitted directly from the community to the ICU and patients transferred from a referral hospital, as well as from HCWs, and the ICU environment. Patients were sampled every third day during hospitalization. Antibiotic susceptibility testing was performed according to EUCAST guidelines. Pulsed-field gel electrophoresis and multilocus sequence typing were used to determine the genetic relatedness of a subset of MRSE isolates.

Results: We identified 620 MRSE isolates from 570 cultures obtained from 37 HCWs, 14 patients, and 14 environmental surfaces in the ICU. HA-MRSE genotypes were identified at admission in only one of the nine patients admitted directly from the community, of which the majority subsequently were colonized by HA-MRSE genotypes within 3 days during hospitalization. Almost all (89%) of HCWs were nasal carriers of HA-MRSE genotypes. Similarly, a significant proportion of patients transferred from the referral hospital and fomites in the ICU were widely colonized with HA-MRSE genotypes.

Conclusions: Patients transferred from a referral hospital, HCWs, and the hospital environment serve as important reservoirs for HA-MRSE. These observations highlight the need for implementation of effective infection prevention and control measures aiming at reducing HA-MRSE transmission in the healthcare setting.

Place, publisher, year, edition, pages
2016. Vol. 16, 743
Keyword [en]
Staphylococcus epidermidis, Cross infection/epidemiology, Cross infection/infection & control, Pulsed-field gel electrophoresis (PFGE), Molecular epidemiology, Multilocus sequence typing (MLST), Healthcare-associated infections, Infectious Disease Transmission, Professional-to-Patient, Intensive Care Units, Environmental Microbiology
National Category
Infectious Medicine Anesthesiology and Intensive Care
Identifiers
URN: urn:nbn:se:umu:diva-130229DOI: 10.1186/s12879-016-2094-xISI: 000390270900002OAI: oai:DiVA.org:umu-130229DiVA: diva2:1065836
Available from: 2017-01-16 Created: 2017-01-14 Last updated: 2017-01-16Bibliographically approved

Open Access in DiVA

fulltext(1022 kB)46 downloads
File information
File name FULLTEXT01.pdfFile size 1022 kBChecksum SHA-512
ae0e67896727a2013f6f20ecb2008fe2b2ce1d7217523e57f7baad749d81c3328bd8df25f4d3b14de8fb3226c09ad61a6d2b58b0bd60cea72d9b5c02a3350167
Type fulltextMimetype application/pdf

Other links

Publisher's full text

Search in DiVA

By author/editor
Widerström, MicaelWiström, JohanEdebro, HelénMarklund, ElisabethBackman, MattiasLindqvist, PerMonsen, Tor
By organisation
Department of Clinical MicrobiologyInfectious DiseasesAnaesthesiology
In the same journal
BMC Infectious Diseases
Infectious MedicineAnesthesiology and Intensive Care

Search outside of DiVA

GoogleGoogle Scholar
Total: 46 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 31 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf