umu.sePublications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
HS1BP3 negatively regulates autophagy by modulation of phosphatidic acid levels
Show others and affiliations
2016 (English)In: Nature Communications, ISSN 2041-1723, E-ISSN 2041-1723, Vol. 7, 13889Article in journal (Refereed) Published
Abstract [en]

A fundamental question is how autophagosome formation is regulated. Here we show that the PX domain protein HS1BP3 is a negative regulator of autophagosome formation. HS1BP3 depletion increased the formation of LC3-positive autophagosomes and degradation of cargo both in human cell culture and in zebrafish. HS1BP3 is localized to ATG16L1-and ATG9-positive autophagosome precursors and we show that HS1BP3 binds phosphatidic acid (PA) through its PX domain. Furthermore, we find the total PA content of cells to be significantly upregulated in the absence of HS1BP3, as a result of increased activity of the PA-producing enzyme phospholipase D (PLD) and increased localization of PLD1 to ATG16L1-positive membranes. We propose that HS1BP3 regulates autophagy by modulating the PA content of the ATG16L1-positive autophagosome precursor membranes through PLD1 activity and localization. Our findings provide key insights into how autophagosome formation is regulated by a novel negative-feedback mechanism on membrane lipids.

Place, publisher, year, edition, pages
2016. Vol. 7, 13889
National Category
Cell and Molecular Biology
Identifiers
URN: urn:nbn:se:umu:diva-130221DOI: 10.1038/ncomms13889ISI: 000390284700001OAI: oai:DiVA.org:umu-130221DiVA: diva2:1066077
Available from: 2017-01-17 Created: 2017-01-14 Last updated: 2017-01-17Bibliographically approved

Open Access in DiVA

fulltext(2278 kB)47 downloads
File information
File name FULLTEXT01.pdfFile size 2278 kBChecksum SHA-512
2723972b34724256f673534ad0305ceb2119ef98097bbf5f0b1573de8d5bb3afcd076ccfc971cd4c28fc2bf693b61636676d22dfb258e835eabec43243b80722
Type fulltextMimetype application/pdf

Other links

Publisher's full text

Search in DiVA

By author/editor
Carlsson, Sven R.
By organisation
Department of Medical Biochemistry and Biophysics
In the same journal
Nature Communications
Cell and Molecular Biology

Search outside of DiVA

GoogleGoogle Scholar
Total: 47 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 22 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf