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Rapid movement and transcriptional re-localization of human cohesin on DNA
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2016 (English)In: EMBO Journal, ISSN 0261-4189, E-ISSN 1460-2075, Vol. 35, no 24, 2671-2685 p.Article in journal (Refereed) Published
Abstract [en]

The spatial organization, correct expression, repair, and segregation of eukaryotic genomes depend on cohesin, ring-shaped protein complexes that are thought to function by entrapping DNA. It has been proposed that cohesin is recruited to specific genomic locations from distal loading sites by an unknown mechanism, which depends on transcription, and it has been speculated that cohesin movements along DNA could create three-dimensional genomic organization by loop extrusion. However, whether cohesin can translocate along DNA is unknown. Here, we used single-molecule imaging to show that cohesin can diffuse rapidly on DNA in a manner consistent with topological entrapment and can pass over some DNA-bound proteins and nucleosomes but is constrained in its movement by transcription and DNA-bound CCCTC-binding factor (CTCF). These results indicate that cohesin can be positioned in the genome by moving along DNA, that transcription can provide directionality to these movements, that CTCF functions as a boundary element for moving cohesin, and they are consistent with the hypothesis that cohesin spatially organizes the genome via loop extrusion.

Place, publisher, year, edition, pages
2016. Vol. 35, no 24, 2671-2685 p.
Keyword [en]
cell cycle, cohesin, genome organization, single-molecule TIRF microscopy, transcription
National Category
Biochemistry and Molecular Biology
Identifiers
URN: urn:nbn:se:umu:diva-130454DOI: 10.15252/embj.201695402ISI: 000390948700008PubMedID: 27799150OAI: oai:DiVA.org:umu-130454DiVA: diva2:1068164
Available from: 2017-01-24 Created: 2017-01-20 Last updated: 2017-03-14Bibliographically approved

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Cisneros, David A.
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Molecular Infection Medicine Sweden (MIMS)Department of Molecular Biology (Faculty of Medicine)
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CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf