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Presence of Collagen-Binding S. mutans Strains in Swedish Children of Potential Caries and Systemic Disease Risk
Umeå University, Faculty of Medicine, Department of Odontology.
Umeå University, Faculty of Medicine, Department of Odontology.
2016 (English)Independent thesis Advanced level (professional degree), 20 credits / 30 HE creditsStudent thesis
Abstract [en]

Streptococcus mutans, a bacterium closely linked to dental caries and lately associated with systemic diseases, is divided into four serotypes; c, e, f and k. Belonging to serotypes f or k, as well as possessing the genes cnm or cbm, and having collagen binding abilities, have been appointed as virulence factors for both caries and systemic conditions.

 

The study objective was to identify presence of S. mutans serotypes c, e, f and k and the genotypes cnm and cbm in 12-year old children in Sweden. Furthermore the study aimed to identify cnm+ bacteria as collagen binding. In total, 40 clinical isolates from colonisation-positive children was screened by PCR for serotype, cnm and cbm. The isolates were also evaluated in a microtitre plates-assay concerning collagen binding ability. The cnm gene was further studied concerning collagen binding in three different experimental designs using additionally 5 cnm+, 5 cnm- and a cnm+ wildtype and its cnm-mutant.

 

The result showed that out of the 40 clinical isolates, 4 were positive for either serotype f or k, genotype cnm or cbm, or positive for collagen binding. One of the isolates was both serotype k, cbm+ and bound strongly to collagen. Additionally, the cnm gene was further associated with collagen bindning.

 

We could establish presence of S. mutans isolates with single virulent qualities as well as isolates with multiple, and can thus conclude presence of strains with both potentially increased risk for caries and for systemic diseases.

 

 

 

Place, publisher, year, edition, pages
2016.
National Category
Medical and Health Sciences
Identifiers
URN: urn:nbn:se:umu:diva-131250OAI: oai:DiVA.org:umu-131250DiVA, id: diva2:1073139
External cooperation
VLL
Educational program
Dentistry Programme
Supervisors
Examiners
Available from: 2017-02-09 Created: 2017-02-09 Last updated: 2017-02-09Bibliographically approved

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CiteExportLink to record
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Citation style
  • apa
  • ieee
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Language
  • de-DE
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  • en-US
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  • nn-NB
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  • Other locale
More languages
Output format
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