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Extensive translational regulation during seed germination revealed by polysomal profiling
Department of Molecular Plant Physiology, Utrecht University, 3584 CH Utrecht, the Netherlands; Wageningen Seed Laboratory, Laboratory of Plant Physiology, Wageningen University, 6708 PB Wageningen, the Netherlands.
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2017 (English)In: New Phytologist, ISSN 0028-646X, E-ISSN 1469-8137, Vol. 214, no 1, 233-244 p.Article in journal (Refereed) Published
Abstract [en]

This work investigates the extent of translational regulation during seed germination. The polysome occupancy of each gene is determined by genome-wide profiling of total mRNA and polysome-associated mRNA. This reveals extensive translational regulation during Arabidopsis thaliana seed germination. The polysome occupancy of thousands of individual mRNAs changes to a large extent during the germination process. Intriguingly, these changes are restricted to two temporal phases (shifts) during germination, seed hydration and germination. Sequence features, such as upstream open reading frame number, transcript length, mRNA stability, secondary structures, and the presence and location of specific motifs correlated with this translational regulation. These features differed significantly between the two shifts, indicating that independent mechanisms regulate translation during seed germination. This study reveals substantial translational dynamics during seed germination and identifies development-dependent sequence features and cis elements that correlate with the translation control, uncovering a novel and important layer of gene regulation during seed germination.

Place, publisher, year, edition, pages
2017. Vol. 214, no 1, 233-244 p.
Keyword [en]
Arabidopsis, germination, imbibition, polysomal profiling, ribosome, RNA structure, seedling establishment, translatomics
National Category
Botany
Research subject
biology; Physiological Botany
Identifiers
URN: urn:nbn:se:umu:diva-132004DOI: 10.1111/nph.14355ISI: 000398130300023PubMedID: 27935038OAI: oai:DiVA.org:umu-132004DiVA: diva2:1077808
Available from: 2017-03-01 Created: 2017-03-01 Last updated: 2017-05-23Bibliographically approved

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CiteExportLink to record
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