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An mRNA decapping mutant deficient in P body assembly limits mRNA stabilization in response to osmotic stress
Umeå University, Faculty of Science and Technology, Department of Molecular Biology (Faculty of Science and Technology). (Tracy Nissan)ORCID iD: 0000-0003-3956-7197
Umeå University, Faculty of Science and Technology, Department of Molecular Biology (Faculty of Science and Technology).ORCID iD: 0000-0003-0482-0543
2017 (English)In: Scientific Reports, ISSN 2045-2322, E-ISSN 2045-2322, Vol. 7, 44395- p., 44395Article in journal (Refereed) Published
Abstract [en]

Yeast is exposed to changing environmental conditions and must adapt its genetic program to provide a homeostatic intracellular environment. An important stress for yeast in the wild is high osmolarity. A key response to this stress is increased mRNA stability primarily by the inhibition of deadenylation. We previously demonstrated that mutations in decapping activators (edc3∆ lsm4∆C), which result in defects in P body assembly, can destabilize mRNA under unstressed conditions. We wished to examine whether mRNA would be destabilized in the edc3∆ lsm4∆C mutant as compared to the wild-type in response to osmotic stress, when P bodies are intense and numerous. Our results show that the edc3∆ lsm4∆C mutant limits the mRNA stability in response to osmotic stress, while the magnitude of stabilization was similar as compared to the wild-type. The reduced mRNA stability in the edc3∆ lsm4∆C mutant was correlated with a shorter PGK1 poly(A) tail. Similarly, the MFA2 mRNA was more rapidly deadenylated as well as significantly stabilized in the ccr4∆ deadenylation mutant in the edc3∆ lsm4∆C background. These results suggest a role for these decapping factors in stabilizing mRNA and may implicate P bodies as sites of reduced mRNA degradation.

Place, publisher, year, edition, pages
2017. Vol. 7, 44395- p., 44395
National Category
Natural Sciences
Research subject
Molecular Biology
Identifiers
URN: urn:nbn:se:umu:diva-132468DOI: 10.1038/srep44395OAI: oai:DiVA.org:umu-132468DiVA: diva2:1081720
Funder
Magnus Bergvall Foundation, 2016-01694Carl Tryggers foundation , 15:347Swedish Research Council, 621-2010-4602
Available from: 2017-03-14 Created: 2017-03-14 Last updated: 2017-04-20
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