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Bi-enzymatic biosensor for on-site, fast and reliable electrochemical detection of relevant D-amino acids in bacterial samples
Umeå University, Faculty of Medicine, Molecular Infection Medicine Sweden (MIMS). Umeå University, Faculty of Medicine, Department of Molecular Biology (Faculty of Medicine). Umeå University, Faculty of Medicine, Umeå Centre for Microbial Research (UCMR).
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2017 (English)In: Sensors and actuators. B, Chemical, ISSN 0925-4005, E-ISSN 1873-3077, Vol. 242, 95-101 p.Article in journal (Refereed) Published
Abstract [en]

In this work, a bi-enzymatic biosensor allowed the total content of D-amino acids (DAAs) determination in highly relevant matrices involving bacteria. The strategy is based on the unique coimmobilization of D-amino acid oxidase (DAAO) and horseradish peroxidase (HRP) enzymes onto a multi-walled carbon nanotubes (MWCNTs) and gold nanoparticles (AuNPs) modified screen-printed electrode (SPCE). The greater amount of AuNPs deposited and hence the greater loading of both enzymes was observed when they were deposited after the activation of the carboxylated MWCNTs with EDC/Sulfo-NHS chemistry. These platforms provided a fast (300s) and selective quantification of DAAs with excellent precision (RSD < 5%) and accuracy (Recoveries 100-104%) in bacterial samples. Collectively, the electrochemical bi-enzymatic biosensor become an universal, fast, sensitive and easy-to-use approach to determine total content of DAAs in complex matrices.

Place, publisher, year, edition, pages
2017. Vol. 242, 95-101 p.
Keyword [en]
D-amino acids, Biosensor, By-enzymatic, Vibrio cholera bacteria, Multi-walled carbon nantotubes, Gold nanoparticles
National Category
Analytical Chemistry
Identifiers
URN: urn:nbn:se:umu:diva-132292DOI: 10.1016/j.snb.2016.11.037ISI: 000393267700013OAI: oai:DiVA.org:umu-132292DiVA: diva2:1089343
Available from: 2017-04-19 Created: 2017-04-19 Last updated: 2017-04-19Bibliographically approved

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Cava, Felipe
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Molecular Infection Medicine Sweden (MIMS)Department of Molecular Biology (Faculty of Medicine)Umeå Centre for Microbial Research (UCMR)
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Sensors and actuators. B, Chemical
Analytical Chemistry

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CiteExportLink to record
Permanent link

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Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
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  • asciidoc
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