umu.sePublications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Improvement in carbohydrate and phlorotannin extraction from Macrocystis pyrifera using carbohydrate active enzyme from marine Alternaria sp. as pretreatment
Umeå University, Faculty of Science and Technology, Department of Chemistry.
Show others and affiliations
2017 (English)In: Journal of Applied Phycology, ISSN 0921-8971, E-ISSN 1573-5176, 1-10 p.Article in journal (Refereed) Published
Abstract [en]

The commercial importance of brown seaweed has been increasing over the past decade, especially due to industries interested in the extraction of phycocolloids and, more recently, of polyphenol compounds such as phlorotannins. The objective of this work was to optimize the extraction conditions of carbohydrates and phlorotannins from Macrocystis pyrifera, evaluated enzymatic pretreatment and different parameters of extraction using design of experiment. The optimal conditions upon extraction of the carbohydrates and phlorotannins were determined by means of a pretreatment protocol taking advantage on a carbohydrate active enzyme, followed by an alkaline hydrolysis with 0.5 N NaOH at 100 °C, 180 min, and S/L ratio of 1/20. In order to extract the carbohydrates, the best conditions found for the pretreatment procedure were 37 °C, pH 7.0 for 24 h, and a S/L ratio of 1/10, giving an extraction yield (EY) of 89.67 ± 12.3 wt.%. In turn, for the extraction of phlorotannins, the best conditions identified in terms of the pretreatment were 25 °C, pH 7.0 for 36 h, and a S/L ratio of 1/20, thus giving a yield (EY) of 2.14 ± 0.25 wt.%. Statistical analysis of both processes revealed a maximum EY of 91.24 wt.% for carbohydrates and 3.31 wt.% EY for phlorotannins.

Place, publisher, year, edition, pages
2017. 1-10 p.
Keyword [en]
Brown seaweed, Extraction, Experimental design, Taguchi design, Box-Behnken design
National Category
Chemical Sciences
Identifiers
URN: urn:nbn:se:umu:diva-133928DOI: 10.1007/s10811-017-1141-3OAI: oai:DiVA.org:umu-133928DiVA: diva2:1089920
Available from: 2017-04-21 Created: 2017-04-21 Last updated: 2017-04-21

Open Access in DiVA

No full text

Other links

Publisher's full texthttp://dx.doi.org/10.1007/s10811-017-1141-3

Search in DiVA

By author/editor
Mikkola, Jyri-Pekka
By organisation
Department of Chemistry
In the same journal
Journal of Applied Phycology
Chemical Sciences

Search outside of DiVA

GoogleGoogle Scholar

Altmetric score

Total: 31 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf