umu.sePublications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Human mesenchymal stem cells secrete hyaluronan-coated extracellular vesicles
Institute of Biomedicine, University of Eastern Finland, Kuopio, Finland.
Institute of Biomedicine, University of Eastern Finland, Kuopio, Finland.
Institute of Biomedicine, University of Eastern Finland, Kuopio, Finland.
Institute of Biomedicine, University of Eastern Finland, Kuopio, Finland.
Show others and affiliations
2017 (English)In: Matrix Biology, ISSN 0945-053X, E-ISSN 1569-1802, S0945-053X(17)30096-3Article in journal (Refereed) Epub ahead of print
Abstract [en]

Extracellular vesicles (EVs) secreted by stem cells are potential factors mediating tissue regeneration. They travel from bone marrow stem cells into damaged tissues, suggesting that they can repair tissue injuries without directly replacing parenchymal cells. We have discovered that hyaluronan (HA) synthesis is associated with the shedding of HA-coated EVs. The aim of this study was to test whether bone marrow-derived hMSCs secrete HA-coated EVs. The EVs secreted by MSCs were isolated by differential centrifugation and characterized by nanoparticle tracking analysis. Their morphology and budding mechanisms were inspected by confocal microscopy and correlative light and electron microscopy. Hyaluronan synthesis of hMSCs was induced by lipopolysaccharide and inhibited by RNA interference and 4-methylumbelliferone. It was found that the MSCs have extremely long apical and lateral HA-coated filopodia, typical for cells with an active HA secretion. Additionally, they secreted HA-coated EVs carrying mRNAs for CD44 and all HAS isoforms. The results show that stem cells have a strong intrinsic potential for HA synthesis and EV secretion, and the amount of HA carried on EVs reflects the HA content of the original cells. These results show that the secretion of HA-coated EVs by hMSCs is a general process, that may contribute to many of the mechanisms of HA-mediated tissue regeneration. Additionally, an HA coat on EVs may regulate their interactions with target cells and participate in extracellular matrix remodeling.

Place, publisher, year, edition, pages
Elsevier, 2017. S0945-053X(17)30096-3
Keyword [en]
CD44, extracellular vesicle, filopodia, hyaluronan, hyaluronan synthase, stem cell
National Category
Cell and Molecular Biology
Research subject
cellforskning; Molecular Cellbiology
Identifiers
URN: urn:nbn:se:umu:diva-134766DOI: 10.1016/j.matbio.2017.05.001PubMedID: 28483644OAI: oai:DiVA.org:umu-134766DiVA: diva2:1094801
Available from: 2017-05-11 Created: 2017-05-11 Last updated: 2017-09-11

Open Access in DiVA

No full text

Other links

Publisher's full textPubMed

Search in DiVA

By author/editor
Qu, ChengjuanLammi, MikkoRilla, Kirsi
By organisation
Department of Integrative Medical Biology (IMB)
In the same journal
Matrix Biology
Cell and Molecular Biology

Search outside of DiVA

GoogleGoogle Scholar

Altmetric score

Total: 70 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf