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Enhancing saccharification of cassava stems by starch hydrolysis prior to pretreatment
Umeå University, Faculty of Science and Technology, Department of Chemistry.
Umeå University, Faculty of Science and Technology, Department of Chemistry.
2017 (English)In: Industrial crops and products (Print), ISSN 0926-6690, E-ISSN 1872-633X, Vol. 97, 21-31 p.Article in journal (Refereed) Published
Abstract [en]

Chemical characterization of cassava stems from different origin revealed that glucans accounted for 54-63% of the dry weight, whereas 35-67% of these glucans consisted of starch. The cassava stems were subjected to a saccharification study including starch hydrolysis, pretreatment with either sulfuric acid or 1-ethyl-3-methylimidazolium acetate ([Emim]OAc), and enzymatic hydrolysis of cellulose. Starch hydrolysis prior to pretreatment decreased sugar degradation, improved enzymatic convertibility of cellulose, and increased overall glucan conversion. Glucan recovery after pretreatment of starch-free cassava stems (SFCS) was around 85%, but below 52% when the stems were pretreated under the same conditions without preparatory starch hydrolysis. The total amount of hydrolyzed glucan after cellulose hydrolysis was two-fold higher for pretreated SFCS than for directly pretreated stems. Pretreatment with [Emim]OAc resulted in 20% higher glucan conversion than pretreatment with acid. Pyrolysis-GC/MS, X-ray diffraction, CP/MAS C-13 NMR and FTIR analyses revealed major differences between H2SO4- and [Emim]OAc-pretreated material. 

Place, publisher, year, edition, pages
ELSEVIER SCIENCE BV , 2017. Vol. 97, 21-31 p.
Keyword [en]
Cassava stems, Cellulose hydrolysis, Dilute-acid pretreatment, Ethanol, Ionic liquid
National Category
Chemical Sciences Agricultural Science, Forestry and Fisheries
Identifiers
URN: urn:nbn:se:umu:diva-133522DOI: 10.1016/j.indcrop.2016.11.067ISI: 000394064600003OAI: oai:DiVA.org:umu-133522DiVA: diva2:1095624
Available from: 2017-05-15 Created: 2017-05-15 Last updated: 2017-06-21Bibliographically approved

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Martín, CarlosJönsson, Leif J.
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CiteExportLink to record
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Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf