umu.sePublications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
The effects of thawing on the plasma metabolome: evaluating differences between thawed plasma and multi-organ samples
Umeå University, Faculty of Science and Technology, Department of Chemistry. Karlsruhe Institute of Technology, Karlsruhe, Germany. (Computational Life Science Cluster (CLiC))
Show others and affiliations
2017 (English)In: Metabolomics, ISSN 1573-3882, E-ISSN 1573-3890, Vol. 13, no 6, 66Article in journal (Refereed) Published
Abstract [en]

Introduction: Post-collection handling, storage and transportation can affect the quality of blood samples. Pre-analytical biases can easily be introduced and can jeopardize accurate profiling of the plasma metabolome. Consequently, a mouse study must be carefully planned in order to avoid any kind of bias that can be introduced, in order not to compromise the outcome of the study. The storage and shipment of the samples should be made in such a way that the freeze–thaw cycles are kept to a minimum. In order to keep the latent effects on the stability of the blood metabolome to a minimum it is essential to study the effect that the post-collection and pre-analytical error have on the metabolome. Objectives: The aim of this study was to investigate the effects of thawing on the metabolic profiles of different sample types. Methods: In the present study, a metabolomics approach was utilized to obtain a thawing profile of plasma samples obtained on three different days of experiment. The plasma samples were collected from the tail on day 1 and 3, while retro-orbital sampling was used on day 5. The samples were analysed using gas chromatography time-of-flight mass spectrometry (GC TOF-MS). Results: The thawed plasma samples were found to be characterized by higher levels of amino acids, fatty acids, glycerol metabolites and purine and pyrimidine metabolites as a result of protein degradation, cell degradation and increased phospholipase activity. The consensus profile was thereafter compared to the previously published study comparing thawing profiles of tissue samples from gut, kidney, liver, muscle and pancreas. Conclusions: The comparison between thawed organ samples and thawed plasma samples indicate that the organ samples are more sensitive to thawing, however thawing still affected all investigated sample types.

Place, publisher, year, edition, pages
Springer, 2017. Vol. 13, no 6, 66
Keyword [en]
Mouse, Metabolomics, Plasma, Multi-organ, Freeze-thaw cycle, OPLS-DA
National Category
Biochemistry and Molecular Biology Endocrinology and Diabetes
Identifiers
URN: urn:nbn:se:umu:diva-135201DOI: 10.1007/s11306-017-1196-9ISI: 000401711400001PubMedID: 28473743OAI: oai:DiVA.org:umu-135201DiVA: diva2:1097204
Available from: 2017-05-22 Created: 2017-05-22 Last updated: 2017-06-27Bibliographically approved

Open Access in DiVA

fulltext(1313 kB)27 downloads
File information
File name FULLTEXT01.pdfFile size 1313 kBChecksum SHA-512
8e6862a0371a25c8ef399a097fed8822d30ea4a90c81643487dc4e1140d8e5c6002646c5ed05315d338a4939d9c9569859742f08e7cd0a51d42b9ee2ce75b844
Type fulltextMimetype application/pdf

Other links

Publisher's full textPubMed

Search in DiVA

By author/editor
Torell, FridaTrygg, Johan
By organisation
Department of Chemistry
In the same journal
Metabolomics
Biochemistry and Molecular BiologyEndocrinology and Diabetes

Search outside of DiVA

GoogleGoogle Scholar
Total: 27 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 57 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf