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Chapter 6. Qualitative and quantitative assessment of the activity of the yeast nonsense-mediated mRNA decay pathway.
University of Massachusetts Medical School, Department of Molecular Genetics and Microbiology.
University of Massachusetts Medical School, Department of Molecular Genetics and Microbiology.
University of Massachusetts Medical School, Department of Molecular Genetics and Microbiology.
University of Massachusetts Medical School, Department of Molecular Genetics and Microbiology.
2008 (English)In: Methods in Enzymology, ISSN 0076-6879, E-ISSN 1557-7988, Vol. 449, 127-47 p.Article in journal (Refereed) Published
Abstract [en]

The yeast Saccharomyces cerevisiae provides an ideal model system for elucidation of the molecular mechanisms that regulate the nonsense-mediated mRNA decay (NMD) pathway. This chapter describes an array of molecular biological, genetic, and biochemical tools that facilitate the characterization of transcripts that comprise NMD substrates and provide insights into the roles of the upf/nmd proteins in mRNA decay and translation termination. Examples illustrate the use of these methods in wild-type and NMD-deficient cells to monitor the abundance, structure, and half-lives of nonsense-containing mRNAs, the read through of premature termination codons by the ribosome, and the positioning of ribosomes at or near normal and premature termination codons.

Place, publisher, year, edition, pages
2008. Vol. 449, 127-47 p.
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Biochemistry and Molecular Biology
Identifiers
URN: urn:nbn:se:umu:diva-135239DOI: 10.1016/S0076-6879(08)02406-3PubMedID: 19215756OAI: oai:DiVA.org:umu-135239DiVA: diva2:1098189
Available from: 2017-05-23 Created: 2017-05-23 Last updated: 2017-05-23

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CiteExportLink to record
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