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LEUNIG_HOMOLOG transcriptional co-repressor mediates aluminium sensitivity through PECTIN METHYLESTERASE46-modulated root cell wall pectin methylesterification in Arabidopsis
Umeå University, Faculty of Science and Technology, Umeå Plant Science Centre (UPSC).
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2017 (English)In: The Plant Journal, ISSN 0960-7412, E-ISSN 1365-313X, Vol. 90, no 3, 491-504 p.Article in journal (Refereed) Published
Abstract [en]

A major factor determining aluminium (Al) sensitivity in higher plants is the binding of Al to root cell walls. The Al binding capacity of cell walls is closely linked to the extent of pectin methylesterification, as the presence of methyl groups attached to the pectin backbone reduces the net negative charge of this polymer and hence limits Al binding. Despite recent progress in understanding the molecular basis of Al resistance in a wide range of plants, it is not well understood how the methylation status of pectin is mediated in response to Al stress. Here we show in Arabidopsis that mutants lacking the gene LEUNIG_HOMOLOG (LUH), a member of the Groucho-like family of transcriptional co-repressor, are less sensitive to Al-mediated repression of root growth. This phenotype is correlated with increased levels of methylated pectin in the cell walls of luh roots as well as altered expression of cell wall-related genes. Among the LUH-repressed genes, PECTIN METHYLESTERASE46 (PME46) was identified as reducing Al binding to cell walls and hence alleviating Al-induced root growth inhibition by decreasing PME enzyme activity. seuss-like2 (slk2) mutants responded to Al in a similar way as luh mutants suggesting that a LUH-SLK2 complex represses the expression of PME46. The data are integrated into a model in which it is proposed that PME46 is a major inhibitor of pectin methylesterase activity within root cell walls.

Place, publisher, year, edition, pages
2017. Vol. 90, no 3, 491-504 p.
Keyword [en]
Arabidopsis thaliana, aluminium, cell wall, LEUNIG_HOMOLOG, pectin methylesterification, pectin, ot growth, LHAIZE E, 1995, PLANT PHYSIOLOGY, V107, P315 ahle Melissa I., 2009, PLANT CELL, V21, P3105 pittke P. M., 2008, PLANT AND SOIL, V303, P217 chian Leon V., 2015, ANNUAL REVIEW OF PLANT BIOLOGY, VOL 66, V66, P571 rkovic O, 2004, CARBOHYDRATE RESEARCH, V339, P2281 chian LV, 2004, ANNUAL REVIEW OF PLANT BIOLOGY, V55, P459 e Joanne E., 2014, PLANT JOURNAL, V80, P122
National Category
Botany
Identifiers
URN: urn:nbn:se:umu:diva-135272DOI: 10.1111/tpj.13506ISI: 000399732200005PubMedID: 28181322OAI: oai:DiVA.org:umu-135272DiVA: diva2:1098797
Available from: 2017-05-26 Created: 2017-05-26 Last updated: 2017-05-26Bibliographically approved

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